Panoutsopoulos Georgios I
Department of Experimental Pharmacology, Medical School, Athens University, 75 Mikras Asias St., Athens 115 27, Greece.
In Vivo. 2004 Nov-Dec;18(6):779-86.
2-Phenylethylamine is an endogenous amine, which acts as a neuromodulator of dopaminergic responses. Exogenous 2-phenylethylamine is found in certain foodstuffs and may cause toxic side-effects in susceptible individuals.
The present investigation examined the metabolism of 2-phenylethylamine to phenylacetic acid, via phenylacetaldehyde, in freshly prepared and cryopreserved liver slices. Additionally, it compared the relative contribution of aldehyde oxidase, xanthine oxidase and aldehyde dehydrogenase by using specific inhibitors for each oxidizing enzyme.
In freshly prepared and cryopreserved liver slices, phenylacetic acid was the main metabolite of 2-phenylethalamine. In freshly prepared liver slices, phenylacetic acid was completely inhibited by disulfiram (inhibitor of aldehyde dehydrogenase), whereas isovanillin (inhibitor of aldehyde oxidase) inhibited acid formation to a lesser extent and allopurinol (inhibitor of xanthine oxidase) had no effect. In cryopreserved liver slices, isovanillin inhibited phenylacetic acid by 85%, whereas disulfiram inhibited acid formation to a lesser extent and allopurinol had no effect.
In liver slices, 2-phenylethylamine is rapidly oxidized to phenylacetic acid, via phenylacetaldehyde, by aldehyde dehydrogenase and aldehyde oxidase with no contribution from xanthine oxidase.
2-苯乙胺是一种内源性胺，作为多巴胺能反应的神经调节剂。外源性2-苯乙胺存在于某些食品中，可能会对易感个体产生毒副作用。
本研究检测了新鲜制备和冷冻保存的肝切片中2-苯乙胺通过苯乙醛代谢为苯乙酸的情况。此外，通过使用每种氧化酶的特异性抑制剂，比较了醛氧化酶、黄嘌呤氧化酶和醛脱氢酶的相对贡献。
在新鲜制备和冷冻保存的肝切片中，苯乙酸是2-苯乙胺的主要代谢产物。在新鲜制备的肝切片中，双硫仑（醛脱氢酶抑制剂）完全抑制了苯乙酸的生成，而异香草醛（醛氧化酶抑制剂）对酸生成的抑制作用较小，别嘌呤醇（黄嘌呤氧化酶抑制剂）则无作用。在冷冻保存的肝切片中，异香草醛抑制苯乙酸生成达85%，而双硫仑对酸生成的抑制作用较小，别嘌呤醇无作用。
在肝切片中，2-苯乙胺通过苯乙醛迅速被醛脱氢酶和醛氧化酶氧化为苯乙酸，黄嘌呤氧化酶不参与此过程。
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