[Effects of flavone from leaves of Diospyros kaki on rat vascular smooth muscle cells proliferation stimulated by native low-density lipoprotein in vitro].

OBJECTIVE

To observe whether rat vascular smooth muscle cells (VSMCs) proliferation induced by native low-density lipoprotein (LDL) is affected by flavone from leaves of Diospyros kaki in vitro.

METHODS

Rat aortic VSMCs were cultured in vitro and treated with LDL and flavone from leaves of Diospyros kaki, respectively, and were observed in comparison with the control group. The ratio of cell proliferation was determined by non-radioactive MTS/PES assay.

RESULTS

Compared with control, flavone from leaves of Diospyros kaki can dose-dependently inhibit LDL-stimulated vascular smooth muscle cells proliferation (P < 0.05).

CONCLUSIONS

The flavone from leaves of Diospyros kaki can inhibit proliferation of rat vascular smooth muscle cells exposed to high levels of native LDL. Flavone from leaves of Diospyros kaki may exert vascular protection by inhibiting vascular smooth muscle cell growth associated with hypercholesterolemia.