S100B在环磷酸腺苷诱导的星形胶质细胞骨架重塑中的作用：一项在洋地黄皂苷通透细胞中使用TRTK-12的研究。
Involvement of the S100B in cAMP-induced cytoskeleton remodeling in astrocytes: a study using TRTK-12 in digitonin-permeabilized cells.
作者信息
Frizzo Juliana K, Tramontina Ana Carolina, Tramontina Francine, Gottfried Carmem, Leal Rodrigo B, Donato Rosario, Gonçalves Carlos-Alberto
机构信息
Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
出版信息
Cell Mol Neurobiol. 2004 Dec;24(6):833-40. doi: 10.1007/s10571-004-6922-y.
Abstract
- Stellation of astrocytes in culture involves a complex rearrangement of microfilaments, intermediate filaments, and microtubules, which reflects in part the plasticity of these cells observed during development or after injury. 2. An astrocytic calcium-binding protein, S100B, has been implicated in the regulation of plasticity due to its ability to interact with cytoskeletal proteins. 3. We used digitonin-permeabilized astrocytes to introduce TRTK-12, a peptide that binds to the C-terminal of S100B and blocks its interaction with cytoskeletal proteins. 4. TRTK-12 was able to block cAMP-induced astrocyte stellation and this effect was dependent on the concentration of the peptide. These results support the idea that S100B has a modulatory role on astrocyte morphology.
摘要
- 培养的星形胶质细胞形成星状体涉及微丝、中间丝和微管的复杂重排，这部分反映了这些细胞在发育过程中或损伤后所观察到的可塑性。2. 一种星形胶质细胞钙结合蛋白S100B，因其与细胞骨架蛋白相互作用的能力，而被认为参与了可塑性的调节。3. 我们使用洋地黄皂苷通透处理的星形胶质细胞来导入TRTK-12，这是一种能与S100B的C末端结合并阻断其与细胞骨架蛋白相互作用的肽。4. TRTK-12能够阻断cAMP诱导的星形胶质细胞形成星状体，且这种效应取决于该肽的浓度。这些结果支持了S100B对星形胶质细胞形态具有调节作用这一观点。
相似文献
1
Involvement of the S100B in cAMP-induced cytoskeleton remodeling in astrocytes: a study using TRTK-12 in digitonin-permeabilized cells.
Cell Mol Neurobiol. 2004 Dec;24(6):833-40. doi: 10.1007/s10571-004-6922-y.
2
S100B-mediated inhibition of the phosphorylation of GFAP is prevented by TRTK-12.
Neurochem Res. 2004 Apr;29(4):735-40. doi: 10.1023/b:nere.0000018844.51009.40.
3
The effects of CapZ peptide (TRTK-12) binding to S100B-Ca2+ as examined by NMR and X-ray crystallography.
J Mol Biol. 2010 Mar 12;396(5):1227-43. doi: 10.1016/j.jmb.2009.12.057. Epub 2010 Jan 4.
4
S100B protein stimulates calcineurin activity.
Neuroreport. 2004 Feb 9;15(2):317-20. doi: 10.1097/00001756-200402090-00021.
5
Target binding to S100B reduces dynamic properties and increases Ca(2+)-binding affinity for wild type and EF-hand mutant proteins.
J Mol Biol. 2012 Oct 26;423(3):365-85. doi: 10.1016/j.jmb.2012.07.011. Epub 2012 Jul 21.
6
GFAP phosphorylation studied in digitonin-permeabilized astrocytes: standardization of conditions.
Brain Res. 2000 Jan 17;853(1):32-40. doi: 10.1016/s0006-8993(99)02224-6.
7
Effects of S100A1 and S100B on microtubule stability. An in vitro study using triton-cytoskeletons from astrocyte and myoblast cell lines.
Neuroscience. 2000;99(4):773-83. doi: 10.1016/s0306-4522(00)00238-4.
8
Digitonin-permeabilization of astrocytes in culture monitored by trypan blue exclusion and loss of S100B by ELISA.
Brain Res Brain Res Protoc. 2000 Nov;6(1-2):86-90. doi: 10.1016/s1385-299x(00)00041-6.
9
Solution NMR structure of S100B bound to the high-affinity target peptide TRTK-12.
J Mol Biol. 2002 Dec 13;324(5):1003-14. doi: 10.1016/s0022-2836(02)01152-x.
10
Glutamate uptake is stimulated by extracellular S100B in hippocampal astrocytes.
Cell Mol Neurobiol. 2006 Feb;26(1):81-6. doi: 10.1007/s10571-006-9099-8.
引用本文的文献
1
Simultaneous inhibition of key growth pathways in melanoma cells and tumor regression by a designed bidentate constrained helical peptide.
Biopolymers. 2014 Jul;102(4):344-58. doi: 10.1002/bip.22505.
2
Lactacystin stimulates stellation of cultured rat cortical astrocytes.
Neurochem Res. 2009 May;34(5):859-66. doi: 10.1007/s11064-008-9830-6. Epub 2008 Sep 4.
3
Theoretical study on binding of S100B protein.
J Mol Model. 2007 Nov;13(11):1123-31. doi: 10.1007/s00894-007-0231-6. Epub 2007 Aug 23.
4
Expression of S100B during embryonic development of the mouse cerebellum.
BMC Dev Biol. 2007 Mar 15;7:17. doi: 10.1186/1471-213X-7-17.
本文引用的文献
1
The influence of the extracellular matrix on the morphology and intracellular pH of cultured astrocytes exposed to media lacking bicarbonate.
Neuroscience. 2003;121(3):553-62. doi: 10.1016/s0306-4522(03)00557-8.
2
Molecular mechanisms of S100-target protein interactions.
Microsc Res Tech. 2003 Apr 15;60(6):552-9. doi: 10.1002/jemt.10297.
3
Anatomical aspects of glia-synapse interaction: the perisynaptic glial sheath consists of a specialized astrocyte compartment.
J Physiol Paris. 2002 Apr-Jun;96(3-4):177-82. doi: 10.1016/s0928-4257(02)00004-9.
4
The zinc- and calcium-binding S100B interacts and co-localizes with IQGAP1 during dynamic rearrangement of cell membranes.
J Biol Chem. 2002 Dec 20;277(51):49998-50007. doi: 10.1074/jbc.M205363200. Epub 2002 Oct 10.
5
The C-terminus and linker region of S100B exert dual control on protein-protein interactions with TRTK-12.
Biochemistry. 2002 Apr 30;41(17):5421-8. doi: 10.1021/bi011732m.
6
Rapid morphological changes in astrocytes are accompanied by redistribution but not by quantitative changes of cytoskeletal proteins.
Glia. 2001 Oct;36(1):102-15. doi: 10.1002/glia.1099.
7
S100: a multigenic family of calcium-modulated proteins of the EF-hand type with intracellular and extracellular functional roles.
Int J Biochem Cell Biol. 2001 Jul;33(7):637-68. doi: 10.1016/s1357-2725(01)00046-2.
8
Dynamic signaling between astrocytes and neurons.
Annu Rev Physiol. 2001;63:795-813. doi: 10.1146/annurev.physiol.63.1.795.
9
Immunocontent and secretion of S100B in astrocyte cultures from different brain regions in relation to morphology.
FEBS Lett. 2000 Dec 15;486(3):203-7. doi: 10.1016/s0014-5793(00)02301-2.
10
Focal adhesions: a nexus for intracellular signaling and cytoskeletal dynamics.
Exp Cell Res. 2000 Nov 25;261(1):25-36. doi: 10.1006/excr.2000.5043.
Zotero 插件