Niccoli-Filho Walter, Penna Luiz Alberto Plácido, Rode Sigmar Mello, Riva Rudimar
Academic Group Studies and Research with Lasers in Dentistry, Sao Paulo State University School of Dentistry, Sao Jose dos Campos, Sao Paulo, Brazil.
Photomed Laser Surg. 2004 Dec;22(6):494-8. doi: 10.1089/pho.2004.22.494.
A morphological and ultra-structural study of copper vapor laser (lambda = 510.6 nm) effects on enamel and dentine was performed to show the effects of this radiation.
A total of 15 human molars were cut in half; 15 pieces were separated for irradiation on enamel and 15 for dentine. These two groups were further divided into five experimental groups, including a control group, comprised of three half-sections each, irradiated by a CVL laser with a power of 7 W, a repetition rate of 15,000 pulses/sec and exposed at 500, 600, and 800 msec and 1 sec irradiation times with a 5-sec interval between irradiations.
In an ultra-structural SEM exam, we observed that on the enamel surfaces irradiated for 1 sec there was morphological alteration that consisted of catering, flaking, and melting on the surfaces. There was no alteration for the other exposure times. On the dentine teeth irradiated for 1 sec, we observed an evident ultra-structural alteration of melted tissue and loss of morphological characteristics. In the dentine group irradiated by 800 msec, we observed ablation and a partial loss of morphological characteristics. In the dentine groups irradiated by 500 and 600 msec, no alteration was observed.
The results showed that irradiation with CVL promoted morphologic changes in the enamel as well as in the dentine and demonstrated a need for future studies in order to establish a safe protocol for further use in the odontological practice.
对铜蒸气激光(波长=510.6纳米)对牙釉质和牙本质的影响进行形态学和超微结构研究,以展现这种辐射的作用。
将15颗人类磨牙切成两半;15块用于牙釉质照射,15块用于牙本质照射。这两组又进一步分为五个实验组,包括一个对照组,每组由三个半切片组成,用功率为7瓦、重复频率为15000脉冲/秒的铜蒸气激光照射,照射时间分别为500、600和800毫秒以及1秒,照射间隔为5秒。
在超微结构扫描电子显微镜检查中,我们观察到,照射1秒的牙釉质表面出现形态改变,包括表面出现凹坑、剥落和熔化。其他照射时间未出现改变。照射1秒的牙本质牙齿,我们观察到组织熔化的明显超微结构改变和形态特征丧失。在照射800毫秒的牙本质组中,我们观察到消融和部分形态特征丧失。在照射500和600毫秒的牙本质组中,未观察到改变。
结果表明,铜蒸气激光照射促进了牙釉质和牙本质的形态变化,并表明有必要进行进一步研究,以便制定一个安全方案,供牙科学实践中进一步使用。