[γ-干扰素和白细胞介素-10在克罗恩病和溃疡性结肠炎中的结肠表达]

[Colonic expression of gamma-interferon and interleukin-10 in Crohn's disease and ulcerative colitis].

作者信息

Kallel L, Boubaker J, Louzir H, Ben Ahmed M, Sassi A, Boubaker S, Zouari B, Filali A

机构信息

Service de gastroentérologie A, Hôpital la Rabta, Tunis, Tunisie.

出版信息

Presse Med. 2005 Jan 15;34(1):8-12. doi: 10.1016/s0755-4982(05)83876-x.

DOI:10.1016/s0755-4982(05)83876-x

PMID:15685091

Abstract

OBJECTIVE

The aim of this study was to investigate mucosal expression of INF-gamma and IL-10 in patients with Crohn's disease (CD) or ulcerative colitis (UC).

METHODS

Fourteen patients with CD and 11 patients with ulcerative colitis participated and 7 healthy subjects were also included. Study of the mucosal expression of INF-gamma and IL-10 was conducted using biopsies from healthy and damaged colons, using the inverse transcription and genetic amplification (RT-PCR) technique in real time (Taqman). Our results were expressed as ratio between messenger cytokine (mRNA) levels and ribosomal RNA level of a reference molecule (rRNA 18S), then multiplied by 108.

RESULTS

In the cases of Crohn's disease, the mucosa expressed increased INF-gamma and IL-10 compared with controls (respective medians: 23.03 vs. 1.87 p=0.04 and 20.61 vs. 2.13 p=0.08). A strong positive correlation was found in the mucosal expression of IL-10 and INF-gamma during CD (r=0.9 p<0.0001). In contrast, in patients with UC, the expression of INF-gamma and IL-10 were comparable to those observed in the controls (7.18 vs. 2.18 p=0.36 and 3.66 vs. 1.87 p=0.44).

CONCLUSION

During Crohn's disease, the expression of both IL-10 and INF-gamma was increased and strongly correlated, compared with the controls.

摘要

目的

本研究旨在调查克罗恩病（CD）或溃疡性结肠炎（UC）患者中γ干扰素（INF-γ）和白细胞介素-10（IL-10）的黏膜表达情况。

方法

14例克罗恩病患者和11例溃疡性结肠炎患者参与研究，另纳入7名健康受试者。采用实时逆转录和基因扩增（RT-PCR）技术（Taqman法），对取自健康结肠和病变结肠的活检组织进行INF-γ和IL-10黏膜表达研究。我们的结果表示为信使细胞因子（mRNA）水平与参考分子（18S核糖体RNA，rRNA）的核糖体RNA水平之比，然后乘以108。

结果

在克罗恩病病例中，与对照组相比，黏膜中INF-γ和IL-10表达增加（各自中位数：23.03对1.87，p=0.04；20.61对2.13，p=0.08）。在克罗恩病期间，IL-10和INF-γ的黏膜表达呈强正相关（r=0.9，p<0.0001）。相比之下，在溃疡性结肠炎患者中，INF-γ和IL-10的表达与对照组相当（7.18对2.18，p=0.36；3.66对1.87，p=0.44）。