固相萃取后采用液相色谱-荧光检测法同时分析西酞普兰和去甲基西酞普兰。

Simultaneous analysis of citalopram and desmethylcitalopram by liquid chromatography with fluorescence detection after solid-phase extraction.

作者信息

Meng Qing H, Gauthier Donna

机构信息

Department of Pathology and Molecular Medicine, McMaster University, Ontario, Canada L8N 4A6.

出版信息

Clin Biochem. 2005 Mar;38(3):282-5. doi: 10.1016/j.clinbiochem.2004.12.009.

DOI:10.1016/j.clinbiochem.2004.12.009

PMID:15708552

Abstract

OBJECTIVES

To develop a HPLC method for the determination of citalopram (CIT) and desmethylcitalopram (DCIT).

METHODS

Solid-phase extraction followed by fluorescence detection was used to measure CIT and DCIT in deproteinized plasma.

RESULTS

The extraction recovery for both analytes was 104 +/- 3%. The calibration was linear over the concentration range of 12-1600 ng/mL for CIT and 6-800 ng/mL for DCIT. The within-run CVs were 2.5% for CIT (400 ng/mL) and 2.9% for DCIT (200 ng/mL) and the between-run CVs were 5.2% for CIT and 2.9% for DCIT, respectively. With low concentrations of CIT (50 ng/mL) and DCIT (25 ng/mL), the within-run CVs were 3.1% and 1.1% and the between-run CVs were 7.4% and 8.8%, respectively. The lower limit of quantification was 12 ng/mL for CIT and 6 ng/mL for DCIT.

CONCLUSIONS

This method allows for the simultaneous determination of CIT and DCIT in plasma at therapeutic and toxic drug concentrations.

摘要

目的

建立一种测定西酞普兰（CIT）和去甲基西酞普兰（DCIT）的高效液相色谱法。

方法

采用固相萃取结合荧光检测法测定去蛋白血浆中的CIT和DCIT。

结果

两种分析物的萃取回收率均为104±3%。CIT在12 - 1600 ng/mL浓度范围内、DCIT在6 - 800 ng/mL浓度范围内校准曲线呈线性。日内变异系数（CV）：CIT（400 ng/mL）为2.5%，DCIT（200 ng/mL）为2.9%；日间CV：CIT为5.2%，DCIT为2.9%。低浓度CIT（50 ng/mL）和DCIT（25 ng/mL）时，日内CV分别为3.1%和1.1%，日间CV分别为7.4%和8.8%。CIT的定量下限为12 ng/mL，DCIT为6 ng/mL。