载体两性电解质在电泳中作为潜在缓冲剂的物理化学研究。

Carrier ampholytes as potential buffers in electrophoresis: physico-chemical study.

作者信息

Busnel Jean-Marc, Hennion Marie-Claire, Peltre Gabriel

机构信息

Laboratory of Environment and Analytical Chemistry, UMR CNRS 7121, ESPCI, 10 rue Vauquelin, 75005 Paris, France.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Apr 15;818(1):99-107. doi: 10.1016/j.jchromb.2004.09.036.

DOI:10.1016/j.jchromb.2004.09.036

PMID:15722050

Abstract

Joule heating is a limiting factor when separating proteins in capillary zone electrophoresis (CZE). Low conductivity buffers, are required for high-speed separations. We investigated the use of carrier ampholytes (CA) as background electrolytes (BGE) in CZE. We prepared 25 "narrow pH cuts" of wide pH range (3-10) CA mixture in order to know if these fractions were suitable to be used as BGE in CZE. Each fraction was characterised by CZE analysis, giving an idea of its heterogeneity (number and relative abundance of molecular ampholytes). Conductivities and buffering capacities of each fraction have been also measured. Our conclusion is that "narrow pH cuts" of CA might be well suited buffers for electrophoretic separations.

摘要

在毛细管区带电泳（CZE）中分离蛋白质时，焦耳热是一个限制因素。高速分离需要低电导率缓冲液。我们研究了在CZE中使用载体两性电解质（CA）作为背景电解质（BGE）的情况。我们制备了25个宽pH范围（3 - 10）的CA混合物的“窄pH切割段”，以了解这些组分是否适合用作CZE中的BGE。通过CZE分析对每个组分进行了表征，从而了解其异质性（分子两性电解质的数量和相对丰度）。还测量了每个组分的电导率和缓冲容量。我们的结论是，CA的“窄pH切割段”可能是非常适合电泳分离的缓冲液。