从脑血管出血患者脑脊液中分离出血红蛋白衍生的阿片样肽。

Isolation of a hemoglobin-derived opioid peptide from cerebrospinal fluid of patients with cerebrovascular bleedings.

作者信息

Glämsta E L, Meyerson B, Silberring J, Terenius L, Nyberg F

机构信息

Department of Pharmacology, University of Uppsala, Sweden.

出版信息

Biochem Biophys Res Commun. 1992 Apr 30;184(2):1060-6. doi: 10.1016/0006-291x(92)90699-l.

DOI:10.1016/0006-291x(92)90699-l

PMID:1575724

Abstract

The hemorphins are peptides with opioid activity, which are enzymatically released from hemoglobin. A decapeptide identical to the sequence 32-41 of the beta-, delta-, gamma- or epsilon-chains of hemoglobin has been isolated from human ventricular cerebrospinal fluid (CSF). The peptide, designated LVV-hemorphin-7, was recovered in relatively high amounts (115-300 pmol per ml) from samples of patients with cerebrovascular bleedings, but was not detectable in control CSF. Its identity with the hemoglobin fragment was confirmed by mass spectrometry and gas-phase sequencing.

摘要

血啡肽是具有阿片样活性的肽，由血红蛋白经酶促释放产生。从人脑室脑脊液（CSF）中分离出一种与血红蛋白β、δ、γ或ε链的32 - 41位序列相同的十肽。这种被命名为LVV - 血啡肽 - 7的肽，在脑血管出血患者的样本中以相对较高的量被回收（每毫升115 - 300皮摩尔），但在对照脑脊液中未检测到。通过质谱分析和气相测序证实了它与血红蛋白片段的一致性。