简单青霉脂肪酶的纯化及性质

Purification and properties of lipase from Penicillium simplicissimum.

作者信息

Sztajer H, Lünsdorf H, Erdmann H, Menge U, Schmid R

机构信息

Gesellschaft für Biotechnologische Forschung, Braunschweig, Germany.

出版信息

Biochim Biophys Acta. 1992 Mar 25;1124(3):253-61. doi: 10.1016/0005-2760(92)90137-k.

DOI:10.1016/0005-2760(92)90137-k

PMID:1576166

Abstract

A Penicillium simplicissimum strain has been found to produce an inducible extracellular lipase. Triolein was the best inducer for the enzyme production with the highest activity being achieved after 48 h of incubation. The purified lipase showed a molecular weight of 56,000 by SDS-PAGE. The enzyme exhibited a high ratio of apolar amino acids. The lipase was stable in the pH range of 5-7 and at 50 degrees C for 15 min. The optimum assay conditions were 37 degrees C and pH 5.0. The enzyme showed a high stability in water immiscible organic solvents. Lipase from P. simplicissimum is nonspecific and hydrolyses each of the three bonds of triacylglycerols.

摘要

已发现一种简单青霉菌株可产生一种诱导型胞外脂肪酶。三油酸甘油酯是该酶产生的最佳诱导剂，孵育48小时后活性最高。经SDS-PAGE分析，纯化后的脂肪酶分子量为56,000。该酶表现出高比例的非极性氨基酸。该脂肪酶在pH值5 - 7范围内以及50℃下15分钟内稳定。最佳测定条件为37℃和pH 5.0。该酶在与水不混溶的有机溶剂中表现出高稳定性。简单青霉脂肪酶具有非特异性，可水解三酰甘油的三个键中的每一个。