• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Rapid detection of ofloxacin resistance in Mycobacterium tuberculosis by two low-cost colorimetric methods: resazurin and nitrate reductase assays.通过两种低成本比色法快速检测结核分枝杆菌对氧氟沙星的耐药性：刃天青法和硝酸还原酶法。
J Clin Microbiol. 2005 Apr;43(4):1612-6. doi: 10.1128/JCM.43.4.1612-1616.2005.
2
Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis.检测结核分枝杆菌中利福平耐药性的快速替代方法。
J Antimicrob Chemother. 2004 Jul;54(1):130-3. doi: 10.1093/jac/dkh320. Epub 2004 Jun 9.
3
Evaluation of the resazurin microtiter assay for rapid detection of ofloxacin resistance in M. tuberculosis.用于快速检测结核分枝杆菌对氧氟沙星耐药性的刃天青微量滴定法评估
Int J Tuberc Lung Dis. 2006 Jul;10(7):808-11.
4
Evaluation of nitrate reductase assay for direct detection of drug resistance in Mycobacterium tuberculosis: rapid and inexpensive method for low-resource settings.评估用于直接检测结核分枝杆菌耐药性的硝酸还原酶测定法：适用于资源匮乏地区的快速且廉价的方法。
Indian J Med Microbiol. 2010 Oct-Dec;28(4):363-5. doi: 10.4103/0255-0857.71816.
5
Thin-layer agar for detection of resistance to rifampicin, ofloxacin and kanamycin in Mycobacterium tuberculosis isolates.用于检测结核分枝杆菌分离株对利福平、氧氟沙星和卡那霉素耐药性的薄层琼脂。
Int J Tuberc Lung Dis. 2009 Oct;13(10):1301-4.
6
Comparative evaluation of the nitrate reduction assay, the MTT test, and the resazurin microtitre assay for drug susceptibility testing of clinical isolates of Mycobacterium tuberculosis.用于结核分枝杆菌临床分离株药敏试验的硝酸盐还原试验、MTT 试验和刃天青微量滴定试验的比较评价
J Antimicrob Chemother. 2005 Apr;55(4):500-5. doi: 10.1093/jac/dki023. Epub 2005 Feb 24.
7
Rapid detection of resistance to pyrazinamide in Mycobacterium tuberculosis using the resazurin microtitre assay.利用 Resazurin 微量滴定法快速检测结核分枝杆菌对吡嗪酰胺的耐药性。
J Antimicrob Chemother. 2011 May;66(5):1044-6. doi: 10.1093/jac/dkr057. Epub 2011 Feb 28.
8
Utility of nitrate reductase assay for detection of multidrug-resistant Mycobacterium tuberculosis in a low resource setting.硝酸盐还原酶检测法在资源匮乏地区检测耐多药结核分枝杆菌中的应用
Biomedica. 2011 Jun;31(2):258-63. doi: 10.1590/S0120-41572011000200014.
9
Detection of ofloxacin resistance by nitrate reductase assay in isolates from extrapulmonary tuberculosis.通过硝酸还原酶法检测肺外结核分离株对氧氟沙星的耐药性。 需注意，原英文文本中“Detection of ofloxacin resistance by nitrate reductase assay in isolates from extrapulmonary tuberculosis.”存在一处多余的“of”，正确表述应为“Detection of ofloxacin resistance by nitrate reductase assay in isolates from extrapulmonary tuberculosis.” 。
Indian J Med Microbiol. 2017 Jan-Mar;35(1):69-73. doi: 10.4103/ijmm.IJMM_16_266.
10
Evaluation of the nitrate reductase assay for rapid detection of extensively drug-resistant tuberculosis.评估硝酸还原酶测定法快速检测广泛耐药结核病。
Int J Tuberc Lung Dis. 2009 Dec;13(12):1542-9.

引用本文的文献

1
Rapid Diagnosis of Drug-Resistant Tuberculosis-Opportunities and Challenges.耐药结核病的快速诊断——机遇与挑战
Pathogens. 2023 Dec 27;13(1):27. doi: 10.3390/pathogens13010027.
2
Molecular Epidemiology and Polymorphism Analysis in Drug-Resistant Genes in Clinical Isolates from Western and Northern India.印度西部和北部临床分离株耐药基因的分子流行病学与多态性分析
Infect Drug Resist. 2022 Apr 8;15:1717-1732. doi: 10.2147/IDR.S345855. eCollection 2022.
3
Bedaquiline Drug Resistance Emergence Assessment in Multidrug-Resistant Tuberculosis (MDR-TB): a 5-Year Prospective Surveillance Study of Bedaquiline and Other Second-Line Drug Susceptibility Testing in MDR-TB Isolates.贝达喹啉耐药性在耐多药结核病（MDR-TB）中的出现评估：一项贝达喹啉和其他二线药物耐药性检测在 MDR-TB 分离株中的 5 年前瞻性监测研究。
J Clin Microbiol. 2022 Jan 19;60(1):e0291920. doi: 10.1128/JCM.02919-20. Epub 2021 Oct 27.
4
The Effect of Long-Term Storage on .长期储存对. 的影响。
Pol J Microbiol. 2021 Sep;70(3):327-337. doi: 10.33073/pjm-2021-031. Epub 2021 Sep 17.
5
Complex Formation of Resorufin and Resazurin with Β-Cyclodextrins: Can Cyclodextrins Interfere with a Resazurin Cell Viability Assay?Resorufin 和 Resazurin 与 β-环糊精的络合作用：环糊精会干扰 Resazurin 细胞活力测定吗？
Molecules. 2018 Feb 10;23(2):382. doi: 10.3390/molecules23020382.
6
Comparative evaluation of the Nitrate Reductase Assay and the Resazurin Microtitre Assay for drug susceptibility testing of Mycobacterium tuberculosis against first line anti-tuberculosis drugs.硝酸还原酶试验和 Resazurin 微量稀释法检测一线抗结核药物对结核分枝杆菌药物敏感性的比较评价。
Braz J Microbiol. 2008 Jan;39(1):16-20. doi: 10.1590/S1517-83822008000100004. Epub 2008 Mar 1.
7
Evaluation of MGIT 960 System for the Second-Line Drugs Susceptibility Testing of Mycobacterium tuberculosis.评估MGIT 960系统用于结核分枝杆菌二线药物敏感性检测
Tuberc Res Treat. 2013;2013:108401. doi: 10.1155/2013/108401. Epub 2013 Mar 28.
8
Evaluation of the performance of nitrate reductase assay for rapid drug-susceptibility testing of mycobacterium tuberculosis in north India.印度北部用于结核分枝杆菌快速药敏试验的硝酸还原酶测定性能评估。
J Health Popul Nutr. 2011 Feb;29(1):20-5. doi: 10.3329/jhpn.v29i1.7563.
9
Fluoroquinolone resistance in Mycobacterium tuberculosis and mutations in gyrA and gyrB.结核分枝杆菌中的氟喹诺酮耐药性以及gyrA和gyrB基因的突变
Antimicrob Agents Chemother. 2009 Oct;53(10):4498-500. doi: 10.1128/AAC.00287-09. Epub 2009 Aug 17.
10
Fluoroquinolone resistance in Mycobacterium tuberculosis: an assessment of MGIT 960, MODS and nitrate reductase assay and fluoroquinolone cross-resistance.结核分枝杆菌对氟喹诺酮类药物的耐药性：对MGIT 960、MODS和硝酸还原酶测定以及氟喹诺酮类药物交叉耐药性的评估
J Antimicrob Chemother. 2009 Jun;63(6):1173-8. doi: 10.1093/jac/dkp096. Epub 2009 Mar 28.

本文引用的文献

1
Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis.检测结核分枝杆菌中利福平耐药性的快速替代方法。
J Antimicrob Chemother. 2004 Jul;54(1):130-3. doi: 10.1093/jac/dkh320. Epub 2004 Jun 9.
2
A microplate indicator-based method for determining the susceptibility of multidrug-resistant Mycobacterium tuberculosis to antimicrobial agents.一种基于微孔板指示剂的方法，用于测定耐多药结核分枝杆菌对抗菌药物的敏感性。
Int J Tuberc Lung Dis. 2004 Feb;8(2):253-9.
3
Results of a standardised regimen for multidrug-resistant tuberculosis in Bangladesh.孟加拉国耐多药结核病标准化治疗方案的结果。
Int J Tuberc Lung Dis. 2004 May;8(5):560-7.
4
Fluoroquinolone resistance in patients with newly diagnosed tuberculosis.新诊断肺结核患者的氟喹诺酮耐药情况。
Clin Infect Dis. 2003 Dec 1;37(11):1448-52. doi: 10.1086/379328. Epub 2003 Nov 4.
5
The rapid development of fluoroquinolone resistance in M. tuberculosis.结核分枝杆菌对氟喹诺酮耐药性的快速发展。
N Engl J Med. 2003 Nov 13;349(20):1977-8. doi: 10.1056/NEJM200311133492023.
6
Ofloxacin in multidrug resistant tuberculosis.氧氟沙星在耐多药结核病中的应用
J Indian Med Assoc. 2003 Mar;101(3):210-2.
7
Resazurin microtiter assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs: rapid, simple, and inexpensive method.结核分枝杆菌对二线药物敏感性的刃天青微量滴定板检测：快速、简便且廉价的方法。
Antimicrob Agents Chemother. 2003 Nov;47(11):3616-9. doi: 10.1128/AAC.47.11.3616-3619.2003.
8
MYCOBACTERIA: LABORATORY METHODS FOR TESTING DRUG SENSITIVITY AND RESISTANCE.分枝杆菌：药物敏感性和耐药性检测的实验室方法
Bull World Health Organ. 1963;29(5):565-78.
9
Fluoroquinolones, tuberculosis, and resistance.氟喹诺酮类、结核病与耐药性。
Lancet Infect Dis. 2003 Jul;3(7):432-42. doi: 10.1016/s1473-3099(03)00671-6.
10
The global situation of MDR-TB.耐多药结核病的全球形势。
Tuberculosis (Edinb). 2003;83(1-3):44-51. doi: 10.1016/s1472-9792(02)00058-6.

通过两种低成本比色法快速检测结核分枝杆菌对氧氟沙星的耐药性：刃天青法和硝酸还原酶法。

Rapid detection of ofloxacin resistance in Mycobacterium tuberculosis by two low-cost colorimetric methods: resazurin and nitrate reductase assays.

作者信息

Martin Anandi, Palomino Juan Carlos, Portaels Françoise

机构信息

Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat, 155, Antwerp B-2000, Belgium.

出版信息

J Clin Microbiol. 2005 Apr;43(4):1612-6. doi: 10.1128/JCM.43.4.1612-1616.2005.

DOI:10.1128/JCM.43.4.1612-1616.2005
PMID:15814974
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1081354/
Abstract

We have evaluated the performance of two rapid, low-cost methods for the detection of ofloxacin (OFX) resistance with 95 Mycobacterium tuberculosis isolates from countries with high multidrug-resistant tuberculosis endemicity. Results obtained by nitrate reductase and resazurin assays showed 100% agreement with those of the proportion method on 7H11 agar using 2 mug of OFX/ml. We confirmed the resistance of all isolates found to be resistant to OFX by the Mycobacterium Growth Indicator Tube system, and complete agreement among all methods was observed. Nitrate reductase and resazurin assays are rapid, simple, low-cost methods and might become inexpensive alternative procedures for rapid detection of OFX resistance in low-resource countries.

摘要

我们用来自耐多药结核病高发国家的95株结核分枝杆菌分离株，评估了两种快速、低成本检测氧氟沙星（OFX）耐药性方法的性能。通过硝酸还原酶和刃天青试验获得的结果与在含2μg/ml OFX的7H11琼脂上采用比例法获得的结果显示100%一致。我们通过分枝杆菌生长指示管系统确认了所有被发现对OFX耐药的分离株的耐药性，并且观察到所有方法之间完全一致。硝酸还原酶和刃天青试验是快速、简单、低成本的方法，可能会成为资源匮乏国家快速检测OFX耐药性的廉价替代方法。