神经酰胺以钙依赖的方式诱导拟南芥细胞发生程序性细胞死亡。

Ceramides induce programmed cell death in Arabidopsis cells in a calcium-dependent manner.

作者信息

Townley Helen E, McDonald Kerrie, Jenkins Gareth I, Knight Marc R, Leaver Christopher J

机构信息

Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK.

出版信息

Biol Chem. 2005 Feb;386(2):161-6. doi: 10.1515/BC.2005.020.

DOI:10.1515/BC.2005.020

PMID:15843160

Abstract

While the role of C2-ceramide in the induction of programmed cell death (PCD) in animal systems has been well documented, little is known of its role in plant cells. Here we show that C2-ceramide induces PCD in Arabidopsis suspension cultures, which is preceded by the generation of a calcium transient and an increase in reactive oxygen species (ROS). Inhibition of the calcium transient prevented cell death, whereas inhibition of ROS had no effect on cell survival. These observations suggest that calcium signalling plays a role in ceramide-induced PCD but is independent of the generation of ROS.

摘要

虽然C2-神经酰胺在动物系统中诱导程序性细胞死亡（PCD）的作用已有充分记录，但对其在植物细胞中的作用却知之甚少。我们在此表明，C2-神经酰胺在拟南芥悬浮培养物中诱导PCD，在此之前会产生钙瞬变和活性氧（ROS）增加。抑制钙瞬变可防止细胞死亡，而抑制ROS对细胞存活没有影响。这些观察结果表明，钙信号传导在神经酰胺诱导的PCD中起作用，但与ROS的产生无关。