Zhu Hongdong, Meloni Bruno P, Bojarski Christina, Knuckey Michael W, Knuckey Neville W
Department of Neurosurgery, Sir Charles Gairdner Hospital, Centre for Neuromuscular and Neurological Disorders, University of Western Australia, Australian Neuromuscular Research Institute, Australia.
Exp Neurol. 2005 Jun;193(2):361-8. doi: 10.1016/j.expneurol.2005.01.022.
In this study, we investigated the efficacy of pre- and 2 h post-ischemic magnesium treatment with different durations of modest hypothermia (35 degrees C) induced immediately or 2 h following global cerebral ischemia in rats. In experimental group 1, rats received an intravenous loading dose (LD) of 360 micromol/kg MgSO4 immediately before ischemia followed by a 48 h intravenous infusion (IVI) at 120 micromol/kg/h. Immediately post-ischemia, body temperature was lowered to 35 degrees C for 6 h or maintained at 37 degrees C. In experimental group 2, 2 h after ischemia, rats received the MgSO4 LD/IVI and/or had their body temperature lowered to 35 degrees C for 6, 12 or 24 h. In experimental group 1, ischemic rats receiving 6 h of modest hypothermia demonstrated 9.4% CA1 neuronal survival, whereas rats treated with magnesium alone or magnesium and 6 h of modest hypothermia demonstrated 5.1% and 37.9% neuronal survival, respectively. In experimental group 2, ischemic rats receiving 6, 12 or 24 h of modest hypothermia demonstrated 6.1, 5 and 43% CA1 neuronal survival, respectively. Rats treated with magnesium and 6, 12 or 24 h of modest hypothermia demonstrated 8.1, 9 and 76% neuronal survival, respectively. Our findings demonstrate that post-ischemic treatment with a 24 h duration of modest hypothermia and magnesium is more effective than either treatment used alone.
在本研究中，我们调查了在大鼠全脑缺血即刻或缺血2小时后诱导不同时长适度低温（35摄氏度）情况下，缺血前及缺血后2小时镁治疗的疗效。在实验组1中，大鼠在缺血前即刻接受360微摩尔/千克硫酸镁的静脉负荷剂量（LD），随后以120微摩尔/千克/小时的速度进行48小时静脉输注（IVI）。缺血后即刻，将体温降至35摄氏度持续6小时或维持在37摄氏度。在实验组2中，缺血2小时后，大鼠接受硫酸镁LD/IVI和/或将体温降至35摄氏度持续6、12或24小时。在实验组1中，接受6小时适度低温的缺血大鼠CA1神经元存活率为9.4%，而单独接受镁治疗或镁与6小时适度低温治疗的大鼠神经元存活率分别为5.1%和37.9%。在实验组2中，接受6、12或24小时适度低温的缺血大鼠CA1神经元存活率分别为6.1%、5%和43%。接受镁与6、12或24小时适度低温治疗的大鼠神经元存活率分别为8.1%、9%和76%。我们的研究结果表明，缺血后进行24小时适度低温和镁治疗比单独使用任何一种治疗方法更有效。
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