Konwerski Jamie, Senchuk Megan, Petty Emily, Lahaie David, Schisa Jennifer A
Department of Biology, Central Michigan University, Mount Pleasant, Michigan 48859, USA.
Dev Dyn. 2005 Jul;233(3):1006-12. doi: 10.1002/dvdy.20421.
The Caenorhabditis elegans pos-1 gene encodes a zinc-finger protein that is required for germline specification during embryogenesis. The maternally provided mRNA is translationally regulated both spatially and temporally during early development. We have cloned orthologs of pos-1 from C. briggsae and C. remanei, two Caenorhabditis species that have diverged from C. elegans by approximately 20-40 million years. Two regions in the 3' untranslated region are highly conserved among all three species. We find that the pos-1 RNA is expressed in the hermaphrodite and female gonads of C. briggsae and C. remanei but POS-1 protein is not detected at high levels in C. briggsae until the 2-cell stage of embryogenesis. The protein expression is restricted to the germline precursors of the embryo. We conclude that pos-1 appears to be translationally regulated in C. briggsae as it is in C. elegans and speculate the conserved 3' UTR sequences may be involved.
秀丽隐杆线虫的pos-1基因编码一种锌指蛋白，该蛋白在胚胎发育过程中对于生殖系特化是必需的。母源提供的mRNA在早期发育过程中受到空间和时间上的翻译调控。我们从briggsae线虫和remanei线虫中克隆了pos-1的直系同源基因，这两种秀丽隐杆线虫物种与秀丽隐杆线虫的分化时间约为2000万至4000万年。3'非翻译区的两个区域在所有三个物种中高度保守。我们发现pos-1 RNA在briggsae线虫和remanei线虫的雌雄同体和雌性性腺中表达，但在briggsae线虫胚胎发育的2细胞阶段之前，未检测到高水平的POS-1蛋白。该蛋白表达仅限于胚胎的生殖系前体。我们得出结论，pos-1在briggsae线虫中似乎像在秀丽隐杆线虫中一样受到翻译调控，并推测保守的3' UTR序列可能参与其中。
