从韩国农作物根际分离并鉴定具有促进固氮生长作用的细菌。
Isolation and characterization of diazotrophic growth promoting bacteria from rhizosphere of agricultural crops of Korea.
作者信息
Park Myoungsu, Kim Chungwoo, Yang Jinchul, Lee Hyoungseok, Shin Wansik, Kim Seunghwan, Sa Tongmin
机构信息
Department of Agricultural Chemistry, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
出版信息
Microbiol Res. 2005;160(2):127-33. doi: 10.1016/j.micres.2004.10.003.
Free-living nitrogen fixing bacteria were isolated from rhizosphere of seven different plant namely sesame, maize, wheat, soybean, lettuce, pepper and rice grown in Chungbuk Province, Korea. Five isolates with nitrogenase activity above 150nmol(-1) mg(-1) protein were identified based on, phenotypic and 16S rDNA sequences analysis. The strains were identified as Stenotrophomonas maltophilia (PM-1, PM-26), Bacillus fusiformis (PM-5, PM-24) and Pseudomonas fluorescens (PM-13), respectively. All the isolates produced indole-3-acetic acid (IAA), in the presence of tryptophan, ranging from 100.4 microg ml(-1) (PM-13) to 255 microg ml(-1) (PM-24). The isolate PM-24 (Bacillus fusiformis) exhibiting highest nitrogenase activity (3677.81 nmol h(-1) mg(-1) protein) and IAA production (255microg ml(-1)) has a promising potential for developing as a plant growth promoting rhizobacteria.
从韩国忠清北道种植的七种不同植物（即芝麻、玉米、小麦、大豆、生菜、辣椒和水稻）的根际中分离出了自生固氮菌。基于表型和16S rDNA序列分析，鉴定出了五种固氮酶活性高于150nmol(-1) mg(-1) 蛋白质的分离株。这些菌株分别被鉴定为嗜麦芽窄食单胞菌（PM-1、PM-26）、梭状芽孢杆菌（PM-5、PM-24）和荧光假单胞菌（PM-13）。在存在色氨酸的情况下，所有分离株均产生吲哚-3-乙酸（IAA），产量范围为100.4μg ml(-1)（PM-13）至255μg ml(-1)（PM-24）。表现出最高固氮酶活性（3677.81 nmol h(-1) mg(-1) 蛋白质）和IAA产量（255μg ml(-1)）的分离株PM-24（梭状芽孢杆菌）具有作为植物促生根际细菌开发的潜在前景。
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