小鼠胚胎颅面间充质细胞中Smad 3与Dishevelled之间的相互作用。
Interaction between Smad 3 and Dishevelled in murine embryonic craniofacial mesenchymal cells.
作者信息
Warner D R, Greene R M, Pisano M M
机构信息
Department of Molecular, Cellular, and Craniofacial Biology, University of Louisville Birth Defects Center, Louisville, KY 40292, USA.
出版信息
Orthod Craniofac Res. 2005 May;8(2):123-30. doi: 10.1111/j.1601-6343.2005.00319.x.
OBJECTIVES
To determine the in vivo interaction between Smad 3 and Dishevelled-1.
DESIGN
Cell culture transfection followed by immunoprecipitation with specific antibodies.
SETTING AND SAMPLE POPULATION
The Department of Molecular, Cellular, and Craniofacial Biology, Birth Defects Center, University of Louisville.
EXPERIMENTAL VARIABLE
Overexpression of myc-Smad 3.
OUTCOME MEASURE
Western blotting of anti-Dishevelled immunoprecipitates for Smad 3.
RESULTS
Smad 3 and Dishevelled isoforms-1, -2, and -3 all bind Smad 3 in glutathione-S-transferase (GST) pull-down assays and Smad 3 binds to Dishevelled-1 in vivo. Stimulation of the transforming growth factor beta (TGFbeta) pathway leads to increased binding of Smad 3 and Dishevelled-1 in vivo.
CONCLUSION
Smad 3 binds all three known isoforms of Dishevelled and binds Dishevelled 1 in vivo. TGFbeta signaling modulates the interaction between Smad 3 and Dishevelled-1.
目的
确定Smad 3与Dishevelled-1在体内的相互作用。
设计
细胞培养转染，随后用特异性抗体进行免疫沉淀。
地点和样本群体
路易斯维尔大学分子、细胞与颅面生物学系出生缺陷中心。
实验变量
myc-Smad 3的过表达。
观察指标
针对Smad 3的抗Dishevelled免疫沉淀产物的蛋白质免疫印迹法。
结果
在谷胱甘肽-S-转移酶（GST）下拉试验中，Smad 3与Dishevelled亚型-1、-2和-3均能结合Smad 3，且Smad 3在体内与Dishevelled-1结合。转化生长因子β（TGFβ）信号通路的激活导致Smad 3与Dishevelled-1在体内的结合增加。
结论
Smad 3与Dishevelled的所有三种已知亚型结合，并在体内与Dishevelled 1结合。TGFβ信号传导调节Smad 3与Dishevelled-1之间的相互作用。
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