鸟类血脑屏障处碘甲状腺原氨酸脱碘酶对甲状腺激素可利用性的调节。
Regulation of thyroid hormone availability by iodothyronine deiodinases at the blood-brain barrier in birds.
作者信息
Verhoelst C H J, van der Geyten S, Roelens S A, Darras V M
机构信息
Laboratory of Comparative Endocrinology, Zoological Institute, Katholieke Universiteit Leuven, Belgium.
出版信息
Ann N Y Acad Sci. 2005 Apr;1040:501-3. doi: 10.1196/annals.1327.103.
It is accepted that type II iodothyronine deiodinase (D2) is predominantly found in brain, where it maintains homeostasis of thyroid hormone (TH) levels. The current study describes the production of a polyclonal D2 antiserum and its use in the comparison of D2 protein distribution with that of type I (D1) and type III (D3) deiodinase protein in the chicken choroid plexus (CP). Immunocytochemistry showed high D2 protein expression in the epithelial cells of the CP, whereas the D1 and D3 proteins were absent. Furthermore, dexamethasone treatment led to an upregulation of the D2 protein in these cells.
人们普遍认为，II型碘甲状腺原氨酸脱碘酶（D2）主要存在于大脑中，在那里它维持甲状腺激素（TH）水平的稳态。本研究描述了一种多克隆D2抗血清的制备及其在鸡脉络丛（CP）中D2蛋白分布与I型（D1）和III型（D3）脱碘酶蛋白分布比较中的应用。免疫细胞化学显示CP上皮细胞中D2蛋白表达较高，而D1和D3蛋白缺失。此外，地塞米松处理导致这些细胞中D2蛋白上调。
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