微乳液电动色谱法同时测定穿心莲及中药制剂中的穿心莲内酯和脱水穿心莲内酯

Simultaneous determination of andrographolide and dehydroandrographolide in Andrographis paniculata and Chinese medicinal preparations by microemulsion electrokinetic chromatography.

作者信息

Yanfang Zhao, Xingping Luo, Zongde Zhai, Liren Chen, Yongmin Li

机构信息

Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.

出版信息

J Pharm Biomed Anal. 2006 Jan 23;40(1):157-61. doi: 10.1016/j.jpba.2005.04.003.

DOI:10.1016/j.jpba.2005.04.003

PMID:15894448

Abstract

The present paper describes the development of a microemulsion electrokinetic chromatographic (MEEKC) method for simultaneous determination of andrographolide and dehydroandrographolide in traditional Chinese medicines and Chinese medicinal preparations. The MEEKC method involved the use of sodium dodecyl sulfate (SDS) as surfactant, heptane as organic solvent and butan-1-ol as co-solvent. The effect of temperature and pH of running buffers on separation were examined. The optimized conditions (heptane 0.81% (w/w), SDS 3.31% (w/w), butan-1-ol 6.61% (w/w) and 10mM sodium tetraborate buffer, pH 9.2) allowed a useful and good reproducible separation of the studied analytes to be achieved.

摘要

本文描述了一种微乳液电动色谱（MEEKC）方法的开发，用于同时测定中药和中药制剂中的穿心莲内酯和脱水穿心莲内酯。该MEEKC方法使用十二烷基硫酸钠（SDS）作为表面活性剂，庚烷作为有机溶剂，丁醇作为助溶剂。考察了运行缓冲液的温度和pH对分离的影响。优化条件（庚烷0.81%（w/w），SDS 3.31%（w/w），丁醇6.61%（w/w）和10mM硼酸钠缓冲液，pH 9.2）使得所研究的分析物能够实现有效且重现性良好的分离。