[高盐饮食对Sprague-Dawley大鼠肾脏骨桥蛋白mRNA表达的影响]

[Effect of high salt diet on expression of osteopontin mRNA in Sprague-Dawley rat kidney].

作者信息

Zheng Yong, Liu Zhi-quan, Ye Tao, He Lan, Wang Yi, Fang Yuan, Li Jian-ning, Liu Ping, Ye Feng

机构信息

Department of Cardiology, First Hospital of Xi'an Jiaotong University, Xi'an 710061,China.

出版信息

Di Yi Jun Yi Da Xue Xue Bao. 2005 May;25(5):595-7.

PMID:15897150

Abstract

OBJECTIVE

To investigate the effect of high-salt diet on the expression of osteopontin (OPN) mRNA and its protein in Sprague-Dawley rat kidney.

METHODS

Forty-eight male SD rats aged 10 weeks receiving normal salt diet were enrolled in study, and were divided into two groups and fed with high salt diet (4%NaCl) or normal salt diet (0.6%NaCl) for 12 weeks respectively, with 24 rats in each group. Tail systolic blood pressure and bodyweight were measured in all rats every week. At the end of 4th, 8th and 12th week, 6 rats in each group were sacrificed for detection of the expression of OPN mRNA and its protein in the kidney with quantitatine real-time (QRT)-PCR and immunohistochemistry, respectively.

RESULTS

No significant difference was found in blood pressure, bodyweight and kidney weight/bodyweight between high-salt diet group and normal salt diet group (P>0.05). The expression of OPN mRNA (0.27+/-0.16 vs 0.15+/-0.13, P<0.05) and its protein (0.78+/-0.15 vs 0.61+/-0.11, P<0.01) in SD rat kidney with high-salt diet were up-regulated at the end of 12th week compared with the rats with normal salt diet.

CONCLUSIONS

High-salt diet increase the expression of OPN mRNA and its protein in SD rat kidney. Renal injury induced by high salt intake might be independent of blood pressure change.

摘要

目的

探讨高盐饮食对Sprague-Dawley大鼠肾脏骨桥蛋白（OPN）mRNA及其蛋白表达的影响。

方法

选取48只10周龄雄性SD大鼠，给予正常盐饮食，将其分为两组，分别给予高盐饮食（4%氯化钠）或正常盐饮食（0.6%氯化钠）12周，每组24只。每周测量所有大鼠的尾动脉收缩压和体重。在第4、8和12周结束时，每组处死6只大鼠，分别用定量实时（QRT）-PCR和免疫组化法检测肾脏中OPN mRNA及其蛋白的表达。