Zhao Luhua, Huang Chaoyu, Shan Zhen, Xiang Bingren, Mei Linghua
Analytic Center, China Pharmaceutical University, No. 24 Tongjiaxiang Road, Nanjing, Jiangsu 210009, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Jul 5;821(1):67-74. doi: 10.1016/j.jchromb.2005.04.008.
High-performance liquid chromatography (HPLC) was developed for fingerprint analysis of Psoralea corylifolia. Liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MSn) technique was first employed to identify the components of the fingerprint. The samples were separated with an Alltima C18 column (250 mm x 4.6 mm, 5 microm) by linear gradient elution using water-acetic acid (A; 100:0.1, v/v) and acetonitrile (B; 0 min, 40%; 15 min, 50%; 35 min, 60%; 45 min, 70%; 55 min, 80%; and maintained for 5 min) as mobile phase at a flow rate of 1.0 ml/min and detector wavelength at 245 nm. A standard procedure was developed for HPLC fingerprint analysis. Average chromatogram of 10 batches of P. corylifolia L. from Sichuan and Henan Provinces, PR China, which has been considered as the original and genuine herbal medicine for a long time, was first established as the characteristic fingerprint. There are 12 common peaks in this fingerprint. Ten of these common peaks were identified by MS data. This profile was then used to identify and assess the differences among the herb grown in various areas of China. The HPLC fingerprint analysis is specific and may serve for quality identification and comprehensive evaluation of P. corylifolia.
高效液相色谱法（HPLC）被用于补骨脂指纹图谱分析。首次采用液相色谱 - 电喷雾电离 - 串联质谱（LC - ESI - MSn）技术对指纹图谱中的成分进行鉴定。样品通过Alltima C18柱（250 mm×4.6 mm，5μm）进行分离，采用水 - 乙酸（A；100:0.1，v/v）和乙腈（B；0分钟，40%；15分钟，50%；35分钟，60%；45分钟，70%；55分钟，80%；并保持5分钟）作为流动相，以1.0 ml/min的流速进行线性梯度洗脱，检测波长为245 nm。建立了HPLC指纹图谱分析的标准操作规程。首次将来自中国四川省和河南省的10批补骨脂（长期以来被视为正宗地道药材）的平均色谱图确立为特征指纹图谱。该指纹图谱中有12个共有峰。其中10个共有峰通过质谱数据得到鉴定。然后利用该图谱来鉴别和评估中国不同地区所产药材之间的差异。HPLC指纹图谱分析具有特异性，可用于补骨脂的质量鉴定和综合评价。
