ATX-S10(Na)光动力疗法的细胞周期依赖性疗效

Cell-cycle-dependent efficacy of photodynamic therapy with ATX-S10(Na).

作者信息

Sano Munetaka, Furuta Takahisa, Takahira Kenichiro, Kajimura Masayoshi, Hanai Hiroyuki, Kohno Eiji, Hirano Toru, Hishida Akira

机构信息

First Department of Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu 431-3192, Japan.

出版信息

Lasers Med Sci. 2005;20(1):1-5. doi: 10.1007/s10103-005-0337-y. Epub 2005 May 24.

DOI:10.1007/s10103-005-0337-y

PMID:15912266

Abstract

Photodynamic therapy (PDT) is a useful strategy for treating various cancers. Details of the mechanisms of PDT have not been made clear yet. We intended to study the efficacy of PDT in relation to the cell cycle. HeLa S3 cells were synchronized by the thymidine block method. Cells in different cell cycle phases after release were treated with the water-soluble photosensitizer, ATX-S10(Na). The cellular viability after PDT was determined by the MTT assay. Intracellular levels of ATX-S10(Na) in different cell cycle phases were also determined. We found that cells in the S and G(2)/M phases were hypersensitive to PDT with ATX-S10(Na) in comparison with those in the G(1) phase, and that cellular levels of ATX-S10(Na) were increased in cells in the S and G(2)/M phases compared to those in the G(1) phase. We conclude that cellular ATX-S10(Na) levels differ among the different cell cycle phases, which is associated with the cell-cycle-dependent efficacy of PDT with ATX-S10(Na).

摘要

光动力疗法（PDT）是治疗多种癌症的一种有效策略。PDT的作用机制细节尚未明确。我们旨在研究PDT与细胞周期相关的疗效。采用胸腺嘧啶核苷阻断法使HeLa S3细胞同步化。释放后处于不同细胞周期阶段的细胞用亲水性光敏剂ATX-S10(Na)处理。通过MTT法测定PDT后的细胞活力。还测定了不同细胞周期阶段细胞内ATX-S10(Na)的水平。我们发现，与G(1)期细胞相比，S期和G(2)/M期细胞对含ATX-S10(Na)的PDT高度敏感，并且与G(1)期细胞相比，S期和G(2)/M期细胞内ATX-S10(Na)水平升高。我们得出结论，不同细胞周期阶段的细胞内ATX-S10(Na)水平存在差异，这与含ATX-S10(Na)的PDT的细胞周期依赖性疗效相关。