Wang Xiao-Ping, Liao Jing, Liu Guo-Zhen, Wang Xing-Cui, Shang Hong-Wei
Department of Pathology, Capital University of Medical Sciences, Beijing 100054, China.
World J Gastroenterol. 2005 Jun 21;11(23):3601-4. doi: 10.3748/wjg.v11.i23.3601.
To investigate the co-expression and significance of heat shock protein 70 (HSP70) and glucose-regulated protein 94 (grp94) in human gastric carcinoma cell line BGC-823.
The expression and localization of HSP70 and grp94 in human gastric carcinoma cell line BGC-823 were determined by immunocytochemistry and indirect immunofluorescence cytochemical staining. Flow cytometry was used to analyze the correlation between expression of HSP70, grp94 and cell cycle in BGC-823 cell line.
Gastric cancer cell line BGC-823 expressed high level of HSP70 and grp94. The positive rate of HSP70 and grp94 was 84.9+/-4.94% and 79.6+/-5.16%, respectively. Both of them were stained in cell plasma. There was a significant difference compared with control group (1.9+/-0.94%, P<0.01). During the cell cycle, HSP70 and grp94 were continuously expressed in BGC-823.
HSP70 and grp94 are highly expressed in human gastric carcinoma BGC-823 cells through the whole cell cycle. There is no relationship between expression of HSP70, grp94 and cell cycle.
研究热休克蛋白70(HSP70)和葡萄糖调节蛋白94(grp94)在人胃癌细胞系BGC - 823中的共表达情况及意义。
采用免疫细胞化学和间接免疫荧光细胞化学染色法检测HSP70和grp94在人胃癌细胞系BGC - 823中的表达及定位。运用流式细胞术分析BGC - 823细胞系中HSP70、grp94表达与细胞周期的相关性。
胃癌细胞系BGC - 823高表达HSP70和grp94。HSP70和grp94的阳性率分别为84.9±4.94%和79.6±5.16%。二者均在细胞质中染色。与对照组(1.9±0.94%,P<0.01)相比有显著差异。在细胞周期中,BGC - 823持续表达HSP70和grp94。
HSP70和grp94在人胃癌BGC - 823细胞的整个细胞周期中高表达。HSP70、grp94的表达与细胞周期无关。