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从褐背蟾鱼(Lagocephalus gloveri)皮肤中分离和鉴定胶原蛋白。

Isolation and characterization of collagen from brown backed toadfish (Lagocephalus gloveri) skin.

作者信息

Senaratne L S, Park Pyo-Jam, Kim Se-Kwon

机构信息

Department of Chemistry, Pukyong National University, 599-1, Daeyeon-3-dong, Nam-Gu, Busan 608-737, Republic of Korea.

出版信息

Bioresour Technol. 2006 Jan;97(2):191-7. doi: 10.1016/j.biortech.2005.02.024.

Abstract

Collagen was isolated and characterized from the skin of brown backed toadfish (Lagocephalus gloveri), processing wastes. The total collagen yield extracted was 54.3% on the basis of lyophilized dry weight compared to other vertebrates. According to the electrophoretic pattern, the skin collagen of the fish consisted of heterotrimer alpha1alpha2alpha3. Moreover, the denaturation temperature was 28 degrees C, which was about 9 degrees C lower than that of collagen from porcine skin. Therefore, there is a possibility to use brown backed toadfish skin collagen as an alternative source of collagen for industrial purposes and subsequently it may maximize the economical value of the fish.

摘要

从褐背蟾鱼(Lagocephalus gloveri)的加工废弃物皮肤中分离并鉴定出胶原蛋白。与其他脊椎动物相比,以冻干干重为基础,提取的总胶原蛋白产量为54.3%。根据电泳图谱,该鱼的皮肤胶原蛋白由异源三聚体α1α2α3组成。此外,变性温度为28℃,比猪皮胶原蛋白的变性温度低约9℃。因此,有可能将褐背蟾鱼皮胶原蛋白用作工业用途胶原蛋白的替代来源,随后可使该鱼的经济价值最大化。

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