Retroviral expression screening of oncogenes in natural killer cell leukemia.

作者信息

Choi Young Lim, Moriuchi Ryozo, Osawa Mitsujiro, Iwama Atsushi, Makishima Hideki, Wada Tomoaki, Kisanuki Hiroyuki, Kaneda Ruri, Ota Jun, Koinuma Koji, Ishikawa Madoka, Takada Shuji, Yamashita Yoshihiro, Oshimi Kazuo, Mano Hiroyuki

机构信息

Division of Functional Genomics, Jichi Medical School, 3311-1 Yakushiji, Kawachigun, Tochigi 329-0498, Japan.

出版信息

Leuk Res. 2005 Aug;29(8):943-9. doi: 10.1016/j.leukres.2005.02.002. Epub 2005 Feb 24.

DOI:10.1016/j.leukres.2005.02.002

PMID:15978945

Abstract

Aggressive natural killer cell leukemia (ANKL) is an intractable malignancy that is characterized by the outgrowth of NK cells. To identify transforming genes in ANKL, we constructed a retroviral cDNA expression library from an ANKL cell line KHYG-1. Infection of 3T3 cells with recombinant retroviruses yielded 33 transformed foci. Nucleotide sequencing of the DNA inserts recovered from these foci revealed that 31 of them encoded KRAS2 with a glycine-to-alanine mutation at codon 12. Mutation-specific PCR analysis indicated that the KRAS mutation was present only in KHYG-1 cells, not in another ANKL cell line or in clinical specimens (n=8).

摘要