Site-directed mutagenesis using Pfu DNA polymerase and T4 DNA ligase.
作者信息
Adereth Yair, Champion Kristen J, Hsu Tien, Dammai Vincent
机构信息
Medical University of South Carolina, Charleston, SC, USA.
出版信息
Biotechniques. 2005 Jun;38(6):864, 866, 868. doi: 10.2144/05386BM03.
Abstract
摘要
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Site-directed mutagenesis using Pfu DNA polymerase and T4 DNA ligase.使用Pfu DNA聚合酶和T4 DNA连接酶的定点诱变。
Biotechniques. 2005 Jun;38(6):864, 866, 868. doi: 10.2144/05386BM03.
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本文引用的文献
1
Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h.定点、不依赖连接酶的诱变（SLIM）：一种在4小时内效率接近100%的单管方法。
Nucleic Acids Res. 2004 Dec 7;32(21):e174. doi: 10.1093/nar/gnh172.
2
Versatile PCR-mediated insertion or deletion mutagenesis.通用的聚合酶链式反应介导的插入或缺失诱变
Biotechniques. 2004 Mar;36(3):398-400. doi: 10.2144/04363BM04.
3
A one-tube, two-step polymerase chain reaction-based site-directed mutagenesis method with simple identification of the mutated product.一种基于单管两步聚合酶链反应的定点诱变方法，可简单鉴定突变产物。
Anal Biochem. 2004 Jan 15;324(2):301-3. doi: 10.1016/j.ab.2003.09.009.
4
Site-directed mutagenesis.定点诱变
Methods Enzymol. 1998;293:53-71. doi: 10.1016/s0076-6879(98)93007-5.
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The "megaprimer" method of site-directed mutagenesis.定点诱变的“大引物”方法。
Biotechniques. 1990 Apr;8(4):404-7.
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