梯度洗脱中的峰容量理论。
Theory of peak capacity in gradient elution.
作者信息
Neue Uwe D
机构信息
Waters Corporation, 34 Maple Street, Milford, MA 01757-3696, USA.
出版信息
J Chromatogr A. 2005 Jun 24;1079(1-2):153-61. doi: 10.1016/j.chroma.2005.03.008.
PMID:16038301
Abstract
Peak capacity is the best measure of the performance of a gradient separation. In this paper, the theory of peak capacity for the standard operating conditions of reversed-phase and ion-exchange chromatography is outlined. The influence of the operating conditions on the peak capacity of a separation are discussed. Finally, bandspreading phenomena in gradient chromatography are analyzed.
摘要
峰容量是梯度分离性能的最佳衡量指标。本文概述了反相色谱和离子交换色谱标准操作条件下的峰容量理论。讨论了操作条件对分离峰容量的影响。最后，分析了梯度色谱中的谱带展宽现象。
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