Wu Kangbing, Wang Hong, Chen Fang, Hu Shengshui
Department of Chemistry, Huazhong University of Science and Technology, Wuhan 430074, P.R. China.
Bioelectrochemistry. 2006 May;68(2):144-9. doi: 10.1016/j.bioelechem.2005.05.002. Epub 2005 Jul 25.
A new rapid, convenient and sensitive electrochemical method is described for the determination of procaine in pharmaceutical preparations, based on the unique properties of a multi-wall carbon nanotube (MWNT) thin film. The electrochemical behavior of procaine at the MWNT film-coated glassy carbon electrode (GCE) was investigated in detail, showing that the MWNT-coated GCE exhibits electrocatalytic activity to the oxidation of procaine because of the significant peak current enhancement and the lowering of oxidation overpotential. Furthermore, the mechanism for the oxidation of procaine at the MWNT-coated GCE was also studied. Finally, various experimental parameters such as solution pH value, the amount of MWNT, accumulation conditions and scan rate were optimized for the determination of procaine, and a new method with detection limit of 2 x 10(-7) mol/L was developed for procaine determination. This newly proposed method was successfully demonstrated with procaine hydrochloride injection.
基于多壁碳纳米管（MWNT）薄膜的独特性质，描述了一种用于测定药物制剂中普鲁卡因的新型快速、便捷且灵敏的电化学方法。详细研究了普鲁卡因在MWNT薄膜修饰玻碳电极（GCE）上的电化学行为，结果表明MWNT修饰的GCE对普鲁卡因的氧化具有电催化活性，这是由于显著的峰电流增强和氧化过电位的降低。此外，还研究了普鲁卡因在MWNT修饰GCE上的氧化机理。最后，对溶液pH值、MWNT用量、富集条件和扫描速率等各种实验参数进行了优化以用于普鲁卡因的测定，并开发了一种检测限为2×10⁻⁷ mol/L的测定普鲁卡因的新方法。该新提出的方法已成功应用于盐酸普鲁卡因注射液的检测。
