利巴韦林不是7-甲基鸟苷mRNA帽的功能模拟物。
Ribavirin is not a functional mimic of the 7-methyl guanosine mRNA cap.
作者信息
Yan Yifei, Svitkin Yuri, Lee Joseph M, Bisaillon Martin, Pelletier Jerry
机构信息
Department of Biochemistry, McIntyre Medical Sciences Building, McGill University, Montreal, Québec H3G 1Y6, Canada.
出版信息
RNA. 2005 Aug;11(8):1238-44. doi: 10.1261/rna.2930805.
Abstract
Ribavirin is a guanosine ribonucleoside analog that displays broad-spectrum anti-viral activity and is currently used for the treatment of some viral infections. Ribavirin has recently been proposed to also be a mimic of the 7-methyl guanosine cap found at the 5' end of mRNAs. To obtain supporting functional data for this hypothesis, we assessed the ability of ribavirin triphosphate to interfere with the interaction between eIF4E and 7-methyl guanosine capped mRNA. In chemical cross-linking assays, cap-affinity chromatography, and cap-dependent translation assays, ribavirin was unable to function as a cap analog.
摘要
利巴韦林是一种鸟苷核糖核苷类似物，具有广谱抗病毒活性，目前用于治疗某些病毒感染。最近有人提出，利巴韦林也可模拟位于mRNA 5'端的7-甲基鸟苷帽。为了获得支持这一假说的功能数据，我们评估了三磷酸利巴韦林干扰真核生物翻译起始因子4E（eIF4E）与7-甲基鸟苷帽化mRNA之间相互作用的能力。在化学交联试验、帽亲和色谱法和帽依赖性翻译试验中，利巴韦林均不能作为帽类似物发挥作用。
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