液相色谱-串联质谱法测定禽肉和禽蛋中的硝基呋喃代谢物
Determination of nitrofuran metabolites in poultry muscle and eggs by liquid chromatography-tandem mass spectrometry.
作者信息
Finzi Jane Kelly, Donato Jose Luiz, Sucupira Mauro, De Nucci Gilberto
机构信息
Galeno Research Unit, 1301 Latino Coelho, Parque Taquaral, Campinas, SP 13087-10, Brazil.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Sep 25;824(1-2):30-5. doi: 10.1016/j.jchromb.2005.05.012.
The use of nitrofurans in food-producing animals has been banned in EU. Detection of the protein-bound nitrofuran metabolites is the best approach to evaluate their utilization. A fast, sensitive and reliable LC-MS-MS method is presented to analyze simultaneously the metabolites of four commonly used nitrofuran drugs, furazolidone, furaltadone, nitrofurazone and nitrofurantoin. The sample clean up was performed by a single liquid-liquid extraction step, after a hydrolysis and derivatisation process. Separation of the molecules was performed by liquid chromatography in a C18 column (100 mmx2.1 mm, 4 microm) at room temperature. The quantitative and confirmatory determination of these metabolites was performed by multiple reactions monitoring (MRM). Limits of quantification of 0.5 ngg(-1) were achieved and the total analysis was accomplished in 5 min. This protocol has been applied to identify contaminated samples of poultry muscle and egg products.
欧盟已禁止在食用动物中使用硝基呋喃。检测蛋白质结合的硝基呋喃代谢物是评估其使用情况的最佳方法。本文介绍了一种快速、灵敏且可靠的液相色谱-质谱联用（LC-MS-MS）方法，可同时分析四种常用硝基呋喃药物（呋喃唑酮、呋吗唑酮、呋喃西林和呋喃妥因）的代谢物。在水解和衍生化过程之后，通过单步液-液萃取步骤进行样品净化。分子分离在室温下于C18柱（100 mm×2.1 mm，4 µm）上通过液相色谱进行。这些代谢物的定量和确证测定通过多反应监测（MRM）进行。实现了0.5 ng g⁻¹的定量限，整个分析在5分钟内完成。该方案已应用于鉴定受污染的家禽肌肉和蛋制品样品。
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