[超临界流体萃取法提取干姜提取物的质量标准研究]
[Study on quality standard of extracts from Rhizoma Zingiberis by supercritical fluid extraction].
作者信息
Meng Qing, Feng Yi-fan, Guo Xiao-ling, Chen Geng-fu, Cai Jian-cong
机构信息
Institute of Drug Research, Guangdong College of Pharmacy, Guangzhou 510224, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2005 May;30(10):750-2.
OBJECTIVE
To establish the quality standard of extracts from Rhizoma Zingiberis by supercritical CO2 fluid extraction.
METHOD
The extracts were identified by TLC. The total phenols and the 6-gingerol were determined by dual-wavelength UV spectrophotometry and HPLC separately.
RESULT
The recovery of total phenols was 97.7% (RSD 2.0%). The linear range of 6-gingerol is 0.20-2.0 microg, the average recovery was 97.7% (RSD 2.0%).
CONCLUSION
The method is convenient for a good resolution and can be used for the quality control of extracts from Rhizoma Zingiberis.
目的
建立超临界CO2流体萃取生姜提取物的质量标准。
方法
采用薄层色谱法对提取物进行鉴别。分别采用双波长紫外分光光度法和高效液相色谱法测定总酚和6-姜酚的含量。
结果
总酚的回收率为97.7%（相对标准偏差为2.0%）。6-姜酚的线性范围为0.20 - 2.0微克，平均回收率为97.7%（相对标准偏差为2.0%）。
结论
该方法简便，分离效果好，可用于生姜提取物的质量控制。
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