Mitidieri Sydnei, Souza Martinelli Anne Helene, Schrank Augusto, Vainstein Marilene Henning
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, P.O. Box 15005, CEP 91501-970, Porto Alegre, RS, Brazil.
Bioresour Technol. 2006 Jul;97(10):1217-24. doi: 10.1016/j.biortech.2005.05.022. Epub 2005 Aug 19.
There is a wide range of biotechnological applications for amylases, including the textile, pharmaceutical, food and laundry industries. Hydrolytic enzymes are 100% biodegradable and enzymatic detergents can achieve effective cleaning with lukewarm water. Microorganisms and culture media were tested for amylase production and the best producer was Aspergillus niger L119 (3.9 U ml(-1) +/- 0.2) in submerged culture and its amylase demonstrated excellent activity at 50-55 degrees C and pH 4.0, remaining stable at 53 degrees C for up to 200 h. In order to establish the potential uses of this enzyme in detergents, different formulations were tested using the A. niger amylase extract. Enzyme activity was compared with three commercial formulations. The detergents are used in hospitals to clean surgical and endoscopy equipment. The presence of amylase in the formulation is because of its action within hospital drainage system, whether or not it has any function in cleaning the equipment.
淀粉酶有广泛的生物技术应用，包括纺织、制药、食品和洗衣行业。水解酶100%可生物降解，酶洗涤剂用温水就能有效清洁。对微生物和培养基进行了淀粉酶生产测试，最佳生产者是黑曲霉L119（在深层培养中为3.9 U ml(-1) +/- 0.2），其淀粉酶在50 - 55摄氏度和pH 4.0时表现出优异活性，在53摄氏度下长达200小时仍保持稳定。为确定这种酶在洗涤剂中的潜在用途，使用黑曲霉淀粉酶提取物测试了不同配方。将酶活性与三种商业配方进行了比较。这些洗涤剂用于医院清洁手术和内窥镜设备。配方中存在淀粉酶是因为其在医院排水系统中的作用，无论它在清洁设备方面是否有任何功能。
