[抑制型电导离子色谱法测定植物样品中的植酸盐和磷酸盐]

[Determination of phytate and phosphate in plant samples by suppressed conductivity ion chromatography].

作者信息

Liu Qiaoru, Dong Wenju, Shi Qizeng, Yang Guangrui

机构信息

School of Chemistry and Environmental Sciences, Henan Normal University, Henan Key Laboratory for Environmental Pollution Control, Xinxiang 453007, China.

出版信息

Se Pu. 2005 May;23(3):302-4.

PMID:16124582

Abstract

Determination of phytate and phosphate by suppressed conductivity ion chromatography was investigated. Plant samples were extracted with 1% (mass fraction) trichloroacetic acid (TCA). After centrifugal settling, the supernatant was filtered with a 0.45 microm filter membrane before injected into the ion chromatographic system and detected by suppressed conductivity. The gradient eluent was composed of 0.22 mol/L sodium hydroxide solution, water and 50% (v/v) isopropyl alcohol solution. Phytate and phosphate were determined within 15 min, and no interference was found in the presence of fluorate, chlorate, nitrate, sulphate and TCA. Good linearities for phytate and phosphate were obtained in the ranges of 5-400 mg/L (r2 = 0.9994) and 5-500 mg/L (r2 = 0.9999), and the detection limits (S/N = 3) were 3.5 mg/L and 1.5 mg/L, respectively. The average recoveries for phytate and phosphate were 99.8% and 98.4%, and their relative standard deviations were 1.98% and 2.09%, respectively.

摘要

研究了采用抑制型电导离子色谱法测定植酸盐和磷酸盐。植物样品用1%（质量分数）的三氯乙酸（TCA）进行提取。离心沉降后，上清液用0.45微米滤膜过滤，然后注入离子色谱系统并通过抑制型电导进行检测。梯度洗脱液由0.22 mol/L氢氧化钠溶液、水和50%（v/v）异丙醇溶液组成。植酸盐和磷酸盐在15分钟内即可测定，在存在氟酸盐、氯酸盐、硝酸盐、硫酸盐和TCA的情况下未发现干扰。植酸盐和磷酸盐在5 - 400 mg/L（r2 = 0.9994）和5 - 500 mg/L（r2 = 0.9999）范围内具有良好的线性关系，检测限（S/N = 3）分别为3.5 mg/L和1.5 mg/L。植酸盐和磷酸盐的平均回收率分别为99.8%和98.4%，其相对标准偏差分别为1.98%和2.09%。