苦杏仁苷抑制SNU - C4人结肠癌细胞中与细胞周期相关的基因。
Amygdalin inhibits genes related to cell cycle in SNU-C4 human colon cancer cells.
作者信息
Park Hae-Jeong, Yoon Seo-Hyun, Han Long-Shan, Zheng Long-Tai, Jung Kyung-Hee, Uhm Yoon-Kyung, Lee Je-Hyun, Jeong Ji-Seon, Joo Woo-Sang, Yim Sung-Vin, Chung Joo-Ho, Hong Seon-Pyo
机构信息
Department of Pharmacology, Kohwang Medical Research Institute, College of Pharmacy, Kyung Hee University, Seoul 130-701, South Korea.
出版信息
World J Gastroenterol. 2005 Sep 7;11(33):5156-61. doi: 10.3748/wjg.v11.i33.5156.
AIM
The genes were divided into seven categories according to biological function; apoptosis-related, immune response-related, signal transduction-related, cell cycle-related, cell growth-related, stress response-related and transcription-related genes.
METHODS
We compared the gene expression profiles of SNU-C4 cells between amygdalin-treated (5 mg/mL, 24 h) and non-treated groups using cDNA microarray analysis. We selected genes downregulated in cDNA microarray and investigated mRNA levels of the genes by RT-PCR.
RESULTS
Microarray showed that amygdalin downregulated especially genes belonging to cell cycle category: exonuclease 1 (EXO1), ATP-binding cassette, sub-family F, member 2 (ABCF2), MRE11 meiotic recombination 11 homolog A (MRE11A), topoisomerase (DNA) I (TOP1), and FK506 binding protein 12-rapamycin-associated protein 1 (FRAP1). RT-PCR analysis revealed that mRNA levels of these genes were also decreased by amygdalin treatment in SNU-C4 human colon cancer cells.
CONCLUSION
These results suggest that amygdalin have an anticancer effect via downregulation of cell cycle-related genes in SNU-C4 human colon cancer cells, and might be used for therapeutic anticancer drug.
目的
根据生物学功能将基因分为七类；凋亡相关、免疫反应相关、信号转导相关、细胞周期相关、细胞生长相关、应激反应相关和转录相关基因。
方法
我们使用cDNA微阵列分析比较了苦杏仁苷处理组（5mg/mL，24小时）和未处理组的SNU-C4细胞的基因表达谱。我们选择在cDNA微阵列中下调的基因，并通过RT-PCR研究这些基因的mRNA水平。
结果
微阵列显示苦杏仁苷尤其下调了属于细胞周期类别的基因：核酸外切酶1（EXO1）、ATP结合盒亚家族F成员2（ABCF2）、MRE11减数分裂重组11同源物A（MRE11A）、拓扑异构酶（DNA）I（TOP1）以及FK506结合蛋白12-雷帕霉素相关蛋白1（FRAP1）。RT-PCR分析表明，在SNU-C4人结肠癌细胞中，苦杏仁苷处理也降低了这些基因的mRNA水平。
结论
这些结果表明，苦杏仁苷通过下调SNU-C4人结肠癌细胞中与细胞周期相关的基因发挥抗癌作用，可能用作治疗性抗癌药物。
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