在用于修复大鼠模型右心室心肌缺损的多孔脱细胞牛心包中观察到组织再生。

Tissue regeneration observed in a porous acellular bovine pericardium used to repair a myocardial defect in the right ventricle of a rat model.

作者信息

Chang Yen, Chen Sung-Ching, Wei Hao-Ji, Wu Tsu-Juey, Liang Huang-Chien, Lai Po-Hong, Yang Hang-Hsing, Sung Hsing-Wen

机构信息

Division of Cardiovascular Surgery, Veterans General Hospital-Taichung, and the College of Medicine, National Yang-Ming University, Taipei, Taiwan, Republic of China.

出版信息

J Thorac Cardiovasc Surg. 2005 Sep;130(3):705-11. doi: 10.1016/j.jtcvs.2005.04.007.

DOI:10.1016/j.jtcvs.2005.04.007

PMID:16153917

Abstract

OBJECTIVE

Nonliving synthetic materials have been widely used to repair myocardial defects; however, material-related failures do occur. To overcome these problems, an acellular bovine pericardium with a porous structure fixed with genipin (the AGP patch) was developed.

METHODS

The AGP patch was used to repair a surgically created myocardial defect in the right ventricle of a rat model. A commercially available expanded polytetrafluoroethylene (e-PTFE) patch was used as a control. At retrieval, a computerized mapping system was used to acquire local epicardial electrograms of each implanted sample, and the appearance of each retrieved sample was grossly examined. The retrieved samples were then processed for histologic examination.

RESULTS

The amplitude of local electrograms on the AGP patch increased significantly with increasing implantation duration, whereas only low-amplitude electrograms were observed on the e-PTFE patch throughout the entire course of the study. No aneurysmal dilation of the implanted patches was seen for either studied group. Additionally, no tissue adhesion was observed on the outer (epicardial) surface of the AGP patch, whereas a moderate tissue adhesion was observed on the e-PTFE patch. On the inner (endocardial) surface, intimal thickening was observed for both studied groups; however, no thrombus formation was found. Intact layers of endothelial and mesothelial cells were identified on the inner and outer surfaces of the AGP patch, respectively. At 4 weeks postoperatively, smooth muscle cells, together with neomuscle fibers (with a few neocollagen fibrils), neoglycosaminoglycans, and neocapillaries, were observed to fill the pores in the AGP patch, an indication of tissue regeneration. These observations were more pronounced at 12 weeks postoperatively. In contrast, no apparent tissue regeneration was observed in the e-PTFE patch.

CONCLUSION

The present study indicated that the AGP patch holds promise to become a suitable patch for surgical repair of myocardial defects.

摘要

目的

非生物合成材料已被广泛用于修复心肌缺损；然而，与材料相关的失败情况确实会发生。为克服这些问题，研发了一种用京尼平固定的具有多孔结构的脱细胞牛心包（AGP补片）。

方法

将AGP补片用于修复大鼠模型右心室手术造成的心肌缺损。使用市售的膨体聚四氟乙烯（e-PTFE）补片作为对照。在取出时，使用计算机化标测系统获取每个植入样本的局部心外膜电图，并对每个取出样本的外观进行大体检查。然后对取出的样本进行组织学检查。

结果

AGP补片上局部电图的幅度随植入时间的增加而显著增加，而在整个研究过程中，e-PTFE补片上仅观察到低幅度电图。两个研究组的植入补片均未出现动脉瘤样扩张。此外，在AGP补片的外（心外膜）表面未观察到组织粘连，而在e-PTFE补片上观察到中度组织粘连。在内（心内膜）表面，两个研究组均观察到内膜增厚；然而，未发现血栓形成。在AGP补片的内表面和外表面分别鉴定出完整的内皮细胞层和间皮细胞层。术后4周，观察到平滑肌细胞与新肌纤维（伴有少量新胶原纤维）、新糖胺聚糖和新毛细血管一起填充AGP补片中的孔隙，这表明组织在再生。这些观察结果在术后12周时更为明显。相比之下，在e-PTFE补片中未观察到明显的组织再生。