某些抗高血压药物的液相色谱分离及紫外测定

Liquid chromatographic separation and UV determination of certain antihypertensive agents.

作者信息

Bhushan R, Gupta Deepak, Singh Shravan Kumar

机构信息

Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247 667, India.

出版信息

Biomed Chromatogr. 2006 Feb;20(2):217-24. doi: 10.1002/bmc.557.

DOI:10.1002/bmc.557

PMID:16161185

Abstract

Three antihypertensive agents were extracted and isolated from commercial formulations. These were purified and characterized by melting point, lambdamax and IR. The percentage recovery by extraction process was in the range 81-91%. Active ingredients from binary formulations were separated by RP-HPLC using methanol-water (50:50 v/v) and by TLC using CHCl3-CH3OH (6:1) as mobile phase. Detection was by UV at 210 nm in HPLC, and by iodine vapors in TLC. The solvent conditions from TLC were transferred to open column chromatographic separation. Quantitative determination was carried out using TLC and column chromatography supplemented with UV spectrophotometry. Recovery was in the range 82-93%. Two combination of drugs, viz. amlodipine+ramipril and amlodipine+enalapril, were separated by the three modes of liquid chromatography. The percentage recovery was in the range 80-92% by open column.

摘要

从市售制剂中提取并分离出三种抗高血压药物。通过熔点、最大吸收波长和红外光谱对其进行纯化和表征。提取过程的回收率在81%-91%之间。二元制剂中的活性成分通过以甲醇-水（50:50 v/v）为流动相的反相高效液相色谱法（RP-HPLC）以及以三氯甲烷-甲醇（6:1）为流动相的薄层色谱法（TLC）进行分离。在高效液相色谱中通过210 nm处的紫外光检测，在薄层色谱中通过碘蒸气检测。将薄层色谱的溶剂条件转移至开放柱色谱分离。使用薄层色谱和柱色谱并辅以紫外分光光度法进行定量测定。回收率在82%-93%之间。两种药物组合，即氨氯地平+雷米普利和氨氯地平+依那普利，通过三种液相色谱模式进行分离。开放柱的回收率在80%-92%之间。