嗜热真细菌嗜热栖热菌、芽孢杆菌属和嗜氢菌中的新型线性和支链多胺。

Novel linear and branched polyamines in the extremely thermophilic eubacteria Thermoleophilum, Bacillus and Hydrogenobacter.

作者信息

Hamana K, Niitsu M, Matsuzaki S, Samejima K, Igarashi Y, Kodama T

机构信息

College of Medical Care and Technology, Gunma University, Japan.

出版信息

Biochem J. 1992 Jun 15;284 ( Pt 3)(Pt 3):741-7. doi: 10.1042/bj2840741.

DOI:10.1042/bj2840741

PMID:1622392

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1132601/

Abstract

Novel tertiary branched tetra-amines, quaternary branched penta-amines, linear penta-amines and linear hexa-amines were distributed as the major polyamines in six obligately extremely thermophilic eubacteria belonging to Thermoleophilum, Bacillus or Hydrogenobacter. The major polyamine of Thermoleophilum album and Thermoleophilum minutum was identified as a quaternary branched penta-amine, 4,4-bis(3-aminopropyl)-1,8-diamino-4-azaoctane (NH2[CH2]3N+([CH2]3NH2)2[CH2]4NH2) by h.p.l.c., t.l.c. and g.c.-m.s. Hydrogenobacter thermophilus and Hydrogenobacter halophilus contained another quaternary branched penta-amine, 4,4-bis(3-aminopropyl)-1,7-diamino-4-azaheptane (NH2[CH2]3N([CH2]3NH2)2[CH2]3NH2) as the major polyamine, and tertiary branched tetra-amines (4-(3-aminopropyl)-1,7-diamino-4-azaheptane (NH2[CH2]3N([CH2]3NH2)[CH2]3NH2), 4-(3-aminopropyl)-1,8-diamino-4-azaoctane (NH2[CH2]3N([CH2]3NH2)[CH2]4NH2)) and 4,4-bis(3-aminopropyl)-1,8-diamino-4-azaoctane were confirmed as minor components. Bacillus schlegelii contained a branched tetra-amine, 4-(3-aminopropyl)-1,8-diamino-4-azaoctane, a branched penta-amine, 4,4-bis(3-aminopropyl)-1,8-diamino-4-azaoctane, a linear penta-amine, 1,16-diamino-4,8,13-triazahexadecane (NH2[CH2]3NH[CH2]3NH[CH2]4NH[CH2]3NH2) and linear hexa-amine(s), 1,20-diamino-4,8,12,17-tetra-azaeicosane (NH2[CH2]3NH[CH2]3NH[CH2]3NH[CH2]4NH[CH2]3NH2 ) and/or 1,20-diamino-4,8,13,17-tetra-azaeicosane (NH2[CH2]3NH[CH2]3NH[CH2]4NH[CH2]3NH[CH2]3NH2 ).

摘要

新型叔胺基四胺、季胺基五胺、直链五胺和直链六胺是六种嗜热真细菌（属于嗜热栖热菌属、芽孢杆菌属或嗜氢菌属）中的主要多胺。嗜热栖热菌和微小嗜热栖热菌的主要多胺经高效液相色谱、薄层层析和气相色谱-质谱法鉴定为季胺基五胺，即4,4-双（3-氨丙基）-1,8-二氨基-4-氮杂辛烷（NH2[CH2]3N+([CH2]3NH2)2[CH2]4NH2）。嗜热栖热菌和嗜盐栖热菌含有另一种季胺基五胺，即4,4-双（3-氨丙基）-1,7-二氨基-4-氮杂庚烷（NH2[CH2]3N([CH2]3NH2)2[CH2]3NH2）作为主要多胺，叔胺基四胺（4-（3-氨丙基）-1,7-二氨基-4-氮杂庚烷（NH2[CH2]3N([CH2]3NH2)[CH2]3NH2）、4-（3-氨丙基）-1,8-二氨基-4-氮杂辛烷（NH2[CH2]3N([CH2]3NH2)[CH2]4NH2））和4,4-双（3-氨丙基）-1,8-二氨基-4-氮杂辛烷被确认为次要成分。施氏芽孢杆菌含有一种支链四胺，即4-（3-氨丙基）-1,8-二氨基-4-氮杂辛烷，一种支链五胺，即4,4-双（3-氨丙基）-1,8-二氨基-4-氮杂辛烷，一种直链五胺，即1,16-二氨基-4,8,13-三氮杂十六烷（NH2[CH2]3NH[CH2]3NH[CH2]4NH[CH2]3NH2）和直链六胺，即1,20-二氨基-4,8,12,17-四氮杂二十烷（NH2[CH2]3NH[CH2]3NH[CH2]3NH[CH2]4NH[CH2]3NH2）和/或1,20-二氨基-4,8,13,17-四氮杂二十烷（NH2[CH2]3NH[CH2]3NH[CH2]4NH[CH2]3NH[CH2]3NH2）。