一种用于合成3'-氨基烷基化寡核苷酸的新型氨基化CPG载体。
A novel amino-on CPG-support for the synthesis of 3'-aminoalkylated oligonucleotides.
作者信息
Leuck Michael, Glare Rubina, Zien Nicole, Paul Matthias, Wolter Andreas
机构信息
Proligo Biochemie GmbH, Georg-Heyken-Str. 14, Hamburg 21149, Germany.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2005;24(5-7):989-92. doi: 10.1081/ncn-200059388.
PMID:16248078
Abstract
The synthesis of a novel amino-ON CPG support and its application in the synthesis of 3'-aminoalkylated oligonucleotides is reported. The release of oligonucleotides with free 3'-amino groups is accomplished by treatment with concentrated ammonia for 2 h at 55 degrees C.
摘要
报道了一种新型氨基化CPG载体的合成及其在3'-氨基烷基化寡核苷酸合成中的应用。通过在55℃下用浓氨处理2小时来实现具有游离3'-氨基的寡核苷酸的释放。
Zotero 插件