荧光测定法用于检测鲜牛奶中脱脂奶粉掺假情况。
Use of fluorometry for determination of skim milk powder adulteration in fresh milk.
作者信息
Guan Rong-fa, Liu Dong-hong, Ye Xing-qian, Yang Kai
机构信息
Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310029, China.
出版信息
J Zhejiang Univ Sci B. 2005 Nov;6(11):1101-6. doi: 10.1631/jzus.2005.B1101.
A FAST (fluorescence of advanced Maillard products and Soluble Tryptophan) method for identification of reconstituted milk made from skim milk powder in the fresh milk was developed. Considering milk and skim milk powders variations from different seasons and countries, milk was collected from different dairy farms in different seasons and skim milk powders were collected from different countries to measure the Tryptophan (Trp), advanced Maillard products (AMP) fluorescence values. The results showed that there were differences (P<0.01) between raw and reconstituted milk. The plot of values in each mixed level of raw and reconstituted milk had a correlation coefficient >0.97. The FAST method is a simple, rapid, low-cost and sensitive method enabling the detection of 5% reconstituted milk in fresh milk. The measurement of the Trp, AMP fluorescence values and calculation of the FAST index is a suitable method for large-scale monitoring of fresh milk samples.
开发了一种用于鉴定鲜奶中由脱脂奶粉制成的复原乳的FAST（晚期美拉德产物和可溶性色氨酸荧光）方法。考虑到不同季节和国家的牛奶及脱脂奶粉存在差异，从不同季节的不同奶牛场收集牛奶，并从不同国家收集脱脂奶粉，以测量色氨酸（Trp）、晚期美拉德产物（AMP）的荧光值。结果表明，原料乳和复原乳之间存在差异（P<0.01）。原料乳和复原乳各混合水平下的值的散点图的相关系数>0.97。FAST方法是一种简单、快速、低成本且灵敏的方法，能够检测出鲜奶中5%的复原乳。测量Trp、AMP荧光值并计算FAST指数是大规模监测鲜奶样品的合适方法。
Zotero 插件