共表达人乳头瘤病毒16型L1/L2/E6/E7蛋白的非复制型重组痘苗病毒的构建与鉴定
[Construction and identification of non-replication recombinant vaccinia virus co-expressing human papillomavirus type 16 L1/L2/E6/E7 proteins].
作者信息
Huang Wei, Tian Hou-wen, Ren Jiao, Fan Jiang-tao, Zhao Li, Bian Tao, Lu Zhen-hua, Ruan Li
机构信息
Department of Gynecologic Oncology, The Affiliated Oncology Hospital, Guangxi Medical University, Nanning 530021, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2005 Sep;19(3):240-3.
OBJECTIVE
To generate a human papillomavirus (HPV16) prophylactic and therapeutic vaccine candidate for cervical cancer.
METHODS
HPV16 major capsid protein L1 gene/minor capsid protein L2 gene and HPV16 early E6/E7 genes were inserted into a vaccinia virus expression vector. A strain of non-recombinant vaccinia virus containing the sequences was obtained through a homologous recombination and identified.
RESULTS
DNA hybridization confirmed that the HPV16L1/L2/E6/E7 genes were integrated into vaccinia virus DNA. Western Blot result showed that full-length L1/L2/E6/E7 proteins were co-expressed in CEF cells infected with the recombinant virus.
CONCLUSION
NTVJE6E7CKL1L2 could be taken as a candidate of prophylactic and therapeutic vaccine for HPV-associated tumors and their precancerous transformations.
目的
制备一种用于宫颈癌的人乳头瘤病毒（HPV16）预防性和治疗性候选疫苗。
方法
将HPV16主要衣壳蛋白L1基因/次要衣壳蛋白L2基因以及HPV16早期E6/E7基因插入痘苗病毒表达载体。通过同源重组获得一株含有这些序列的非重组痘苗病毒并进行鉴定。
结果
DNA杂交证实HPV16L1/L2/E6/E7基因已整合到痘苗病毒DNA中。蛋白质免疫印迹结果显示全长L1/L2/E6/E7蛋白在感染重组病毒的鸡胚成纤维细胞（CEF）中共同表达。
结论
NTVJE6E7CKL1L2可作为HPV相关肿瘤及其癌前病变的预防性和治疗性疫苗候选物。
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