Parida B K, Douglas T, Nino C, Dhandayuthapani S
Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA.
Tuberculosis (Edinb). 2005 Sep-Nov;85(5-6):347-55. doi: 10.1016/j.tube.2005.08.001. Epub 2005 Nov 2.
Anti-sigma factor antagonists (anti-anti-sigma factors) play critical roles in regulating the expression of alternative sigma factors in response to specific stress signals. The Clusters of Orthologous Groups (COG) database has identified the existence of six genes, Rv0516c, Rv1364c, Rv1365c, Rv1904, Rv2638 and Rv3687c (grouped under the cluster COG1366), encoding potential anti-sigma factor antagonists in Mycobacterium tuberculosis. These molecules are speculated to regulate the expression of sigma factor SigF of M. tuberculosis in response to stress signals. Since signaling occurs via physical interactions of proteins (protein-protein interaction), we investigated whether the anti-sigma factor antagonists of M. tuberculosis interact with anti-sigma factor RsbW (Rv3287c) or the sigma factor SigF (Rv3286c) in the yeast two-hybrid system. The results revealed that most of the anti-sigma factor antagonists interact with either RsbW or SigF or both. In addition, some anti-sigma factor antagonists also displayed limited interactions between themselves. These interactions suggest that they possibly transduce some signals to SigF and between themselves.
抗西格玛因子拮抗剂（抗抗西格玛因子）在响应特定应激信号调节替代西格玛因子的表达中起关键作用。直系同源簇（COG）数据库已确定存在六个基因，即Rv0516c、Rv1364c、Rv1365c、Rv1904、Rv2638和Rv3687c（归入COG1366簇），它们在结核分枝杆菌中编码潜在的抗西格玛因子拮抗剂。据推测，这些分子在响应应激信号时调节结核分枝杆菌西格玛因子SigF的表达。由于信号传导是通过蛋白质的物理相互作用（蛋白质-蛋白质相互作用）发生的，我们在酵母双杂交系统中研究了结核分枝杆菌的抗西格玛因子拮抗剂是否与抗西格玛因子RsbW（Rv3287c）或西格玛因子SigF（Rv3286c）相互作用。结果显示，大多数抗西格玛因子拮抗剂与RsbW或SigF或两者都相互作用。此外，一些抗西格玛因子拮抗剂之间也表现出有限的相互作用。这些相互作用表明它们可能将一些信号传导给SigF并在它们自身之间传导。
