沙苑子黄酮对大鼠抗肝纤维化的作用。
Effect of Astragalus complanatus flavonoid on anti-liver fibrosis in rats.
作者信息
Liu Chun-Yu, Gu Zhen-Lun, Zhou Wen-Xuan, Guo Ci-Yi
机构信息
Department of Pharmacology, Medical College of Suzhou University, Suzhou 215007, Jiangsu Province, China.
出版信息
World J Gastroenterol. 2005 Oct 7;11(37):5782-6. doi: 10.3748/wjg.v11.i37.5782.
AIM
To observe the anti-liver fibrosis effect of Astragalus complanatus flavonoids (ACF) in rats.
METHODS
The liver fibrosis model in rats was established by injecting interperitoneally 0.2 mL/100 g 0.5% dimethylnitrosamine, thrice a week. Meanwhile, the rats were administered ACF (30, 60, 120 mg/kg) or colchicine (0.1 mg/kg) once a day for 1 mo. Serum N-propeptide of type I procollagen (PINP) and type III procollagen (PIIINP) were measured using ELISA. Malondialdehyde (MDA) and superoxide dismutase (SOD) in hepatic tissue were evaluated. Matrix metal protease-1 (MMP-1) mRNA expression was assayed by RT-PCR and the protein expression of tissue inhibitor of metal protease-1 (TIMP-1) was analyzed by immunohistochemistry.
RESULTS
In the ACF groups, SOD activity increased and MDA content decreased in comparison to the liver fibrosis model group. The serum PINP and PIIINP contents in ACF-2 and -3 group decreased compared to those in model group. In ACF-2 and -3 group, the expression of MMP-1 mRNA increased significantly and the protein expression of TIMP-1 decreased compared to that in model group.
CONCLUSION
The antifibrotic mechanisms of ACF are associated with its influence on lipid peroxidation and collagen synthesis and degradation.
目的
观察沙苑子黄酮（ACF）对大鼠抗肝纤维化的作用。
方法
通过腹腔注射0.2 mL/100 g 0.5%二甲基亚硝胺，每周3次，建立大鼠肝纤维化模型。同时，大鼠每天给予ACF（30、60、120 mg/kg）或秋水仙碱（0.1 mg/kg），持续1个月。采用酶联免疫吸附测定法检测血清I型前胶原氨基端前肽（PINP）和III型前胶原氨基端前肽（PIIINP）。评估肝组织中的丙二醛（MDA）和超氧化物歧化酶（SOD）。通过逆转录-聚合酶链反应检测基质金属蛋白酶-1（MMP-1）mRNA表达，并通过免疫组织化学分析金属蛋白酶组织抑制剂-1（TIMP-1）的蛋白表达。
结果
与肝纤维化模型组相比，ACF各剂量组中SOD活性升高，MDA含量降低。与模型组相比，ACF-2组和ACF-3组血清PINP和PIIINP含量降低。与模型组相比，ACF-2组和ACF-3组MMP-1 mRNA表达显著增加，TIMP-1蛋白表达降低。
结论
ACF的抗纤维化机制与其对脂质过氧化以及胶原合成和降解的影响有关。
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