孔雀石绿琼脂，一种用于镰刀菌属的新型选择性培养基。

Malachite green agar, a new selective medium for Fusarium spp.

作者信息

Castellá G, Bragulat M R, Rubiales M V, Cabañes F J

机构信息

Departament de Patologia i Producció Animals (Microbiologia), Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.

出版信息

Mycopathologia. 1997;137(3):173-8. doi: 10.1023/A:1006886529511.

DOI:10.1023/A:1006886529511

PMID:16283458

Abstract

Malachite Green Agar 2.5 ppm (MGA 2.5) is a potent selective medium for isolation and enumeration of Fusarium spp. It has been tested with pure and mixed cultures as well as in naturally contaminated samples. The recoveries of Fusarium species in MGA 2.5 were the same as the recoveries obtained in Nash and Snyder medium. However, this medium is a more selective culture medium for Fusarium spp. than Nash and Snyder medium, because it does not allow the development of colonies belonging to other fungal genera. MGA 2.5 is simple to prepare and less hazardous than other Fusarium selective media containing pentachloronitrobenzene (PCNB).

摘要

2.5 ppm孔雀石绿琼脂（MGA 2.5）是用于分离和计数镰刀菌属的高效选择性培养基。它已在纯培养物和混合培养物以及天然污染样品中进行了测试。镰刀菌在MGA 2.5中的回收率与在纳什和斯奈德培养基中获得的回收率相同。然而，这种培养基对镰刀菌属的选择性比纳什和斯奈德培养基更强，因为它不允许其他真菌属的菌落生长。MGA 2.5制备简单，且比其他含有五氯硝基苯（PCNB）的镰刀菌选择性培养基危害更小。