[陡脉冲电场对BALB/c小鼠癌细胞致死效应的实验研究]

[Experimental study of the lethal effects of steep pulsed electric field on cancer cells in BALB/c mice].

作者信息

Yao Chenguo, Sun Caixin, Mi Yan, Xiong Lan, Hu Ya, Hu Lina

机构信息

Key Lab of High Voltage Engineering and Electrical New Technology, Ministry of Education, Chongqing University, Chongqing 400044, China.

出版信息

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2005 Oct;22(5):896-900.

PMID:16294716

Abstract

This study was conducted to observe the lethal effects of steep pulsed electric field(SPEF) on the cancerous squamous cells in BALB/c mice. Female mice were inoculated with the solution of cervical cancer cells line. The tumor-bearing mice of the experiment group were exposed to SPEF. Then, the histomorphological changes were examined and compared between experiment group and control group. The histological and antitumor assay showed that SPEF could selectively injure tumor cells and inhibit cell proliferation. After treatment, pyknosis, karyoclasis and karyolysis of cancer cells were observed under light and electron mircroscope. More serious changes appeared some days later. These results indicate that SPEF have lethal effects on cervical cancer. It may prolong the survival period of tumor-bearing mice.

摘要

本研究旨在观察陡脉冲电场（SPEF）对BALB/c小鼠癌性鳞状细胞的致死作用。将雌性小鼠接种宫颈癌细胞系溶液。实验组的荷瘤小鼠暴露于SPEF。然后，检查并比较实验组和对照组的组织形态学变化。组织学和抗肿瘤分析表明，SPEF可选择性损伤肿瘤细胞并抑制细胞增殖。治疗后，在光学显微镜和电子显微镜下观察到癌细胞的核固缩、核碎裂和核溶解。几天后出现更严重的变化。这些结果表明，SPEF对宫颈癌有致死作用。它可能延长荷瘤小鼠的生存期。