儿科重症监护病房中两种用于分离葡萄球菌的血培养程序的比较。

A comparison of two blood culture procedures for the isolation of staphylococci in a paediatric intensive care unit.

作者信息

Belli M L, Ugolotti E, Fenu M L, Mantero E, Ceccarelli R

机构信息

Laboratory for Immunocompromised Host, Gaslini Children's Hospital, Genoa, Italy.

出版信息

Clin Microbiol Infect. 2005 Dec;11(12):1035-7. doi: 10.1111/j.1469-0691.2005.01269.x.

DOI:10.1111/j.1469-0691.2005.01269.x

PMID:16307560

Abstract

Blood culture results obtained between January 2000 and July 2003 were reviewed for 1360 patients in a paediatric intensive care unit (PICU). The BacT/Alert FA aerobic medium was used with a blood volume of 1.5 mL for the first 23 months, and the BacT/Alert PF paediatric medium was used with a 0.5-mL volume for the remaining 18 months. The isolation rates were similar during both periods (13.4% vs. 13.1%), and staphylococci were the most common isolates (72.8%). There was a shorter time to detection of staphylococci with the smaller-volume (PF) procedure, which thus seems suitable for use in the diagnosis of staphylococcal bacteraemia in the PICU.

摘要

对一家儿科重症监护病房（PICU）1360例患者在2000年1月至2003年7月期间获得的血培养结果进行了回顾。在最初的23个月中，使用BacT/Alert FA需氧培养基，血样量为1.5 mL，在随后的18个月中，使用BacT/Alert PF儿科培养基，血样量为0.5 mL。两个时期的分离率相似（13.4%对13.1%），葡萄球菌是最常见的分离菌（72.8%）。采用较小血样量（PF）的方法检测葡萄球菌的时间较短，因此似乎适用于PICU中葡萄球菌菌血症的诊断。