载脂蛋白A1基因多态性与高胆固醇血症个体的血清甘油三酯浓度变化有关。

APOA1 polymorphisms are associated with variations in serum triglyceride concentrations in hypercholesterolemic individuals.

作者信息

Sorkin Simone C, Forestiero Francisco J, Hirata Mario H, Guzmán Elizabeth C R, Cavalli Selma A, Bertolami Marcelo C, Salazar Luis A, Hirata Rosario D C

机构信息

Department of Clinical and Toxicological Analysis, School of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo, Brazil.

出版信息

Clin Chem Lab Med. 2005;43(12):1339-45. doi: 10.1515/CCLM.2005.229.

DOI:10.1515/CCLM.2005.229

PMID:16309370

Abstract

BACKGROUND

Apolipoprotein A-I gene (APOA1) polymorphisms have been associated with variations in serum low-density lipoprotein (LDL)- and high-density lipoprotein (HDL)-cholesterol. We have investigated whether APOA1 common variants are also associated with variations in basal triglyceride serum concentrations and response to atorvastatin in individuals with hypercholesterolemia.

METHODS

APOA1 G-75A and C83T polymorphisms and variations in serum lipids were evaluated in 150 hypercholesterolemic (HC) and 93 normolipidemic (NL) unrelated European-derived Brazilians treated with atorvastatin (10 mg/day for 4 weeks). Genomic DNA was extracted from blood leukocytes using a salting-out method and APOA1 polymorphisms were analyzed by polymerase chain reaction and restriction fragment length polymorphism.

RESULTS

G-75A polymorphism was associated with differences in serum concentrations of triglyceride and very low-density lipoprotein (VLDL)-cholesterol (p=0.026) in HC men. After atorvastatin treatment, women carrying the GG/CC haplotype had lower serum triglyceride and VLDL-cholesterol (p=0.020) than non-carriers. In men, the reduction in serum triglyceride in response to atorvastatin was found to be slightly lower in GG/CC haplotype carriers (p=0.051).

CONCLUSION

Our data suggest that APOA1 polymorphisms are associated with variations of baseline serum concentrations of triglyceride and VLDL-cholesterol and in response to atorvastatin in a gender-specific manner.

摘要

背景

载脂蛋白A-I基因（APOA1）多态性与血清低密度脂蛋白（LDL）和高密度脂蛋白（HDL）胆固醇的变化有关。我们研究了APOA1常见变异是否也与高胆固醇血症患者基础甘油三酯血清浓度的变化以及对阿托伐他汀的反应有关。

方法

对150名接受阿托伐他汀治疗（10毫克/天，共4周）的高胆固醇血症（HC）和93名血脂正常（NL）的非亲缘欧洲裔巴西人进行了APOA1 G-75A和C83T多态性以及血脂变化的评估。采用盐析法从血液白细胞中提取基因组DNA，并通过聚合酶链反应和限制性片段长度多态性分析APOA1多态性。

结果

G-75A多态性与HC男性血清甘油三酯和极低密度脂蛋白（VLDL）胆固醇浓度的差异有关（p=0.026）。阿托伐他汀治疗后，携带GG/CC单倍型的女性血清甘油三酯和VLDL胆固醇水平低于非携带者（p=0.020）。在男性中，发现GG/CC单倍型携带者对阿托伐他汀反应的血清甘油三酯降低幅度略低（p=0.051）。