Determination of theophylline concentration in serum by chemiluminescent immunoassay.

OBJECTIVE

This study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum.

METHODS

To measure the concentration of theophylline (n=122) and evaluate the assay.

RESULTS

The linear range of the CLIA method was 0.51-40 mg/L (Y=1.02X+0.44, r=0.995). The intra and inter CV (coefficient variance) of CLIA were 3.20% and 3.57%, respectively. The average recovery rate was 102.3%. This method was free from interference by brilirubin (<200 micromol/L), hemoglobin (<10 g/L), and triglycerides (<15 mmol/L).

CONCLUSION

This method is simple, convenient and precise for clinical pharmacokinetics study of theophylline.