Jarusiewicz Jamie A, Fried Bernard, Sherma Joseph
Department of Chemistry, Lafayette College, Easton, PA 18042, USA.
Comp Biochem Physiol B Biochem Mol Biol. 2006 Feb;143(2):244-8. doi: 10.1016/j.cbpb.2005.11.014. Epub 2005 Dec 27.
High performance thin layer chromatography was used to quantify the concentrations of beta-carotene and lutein in the whole bodies and digestive gland-gonad complexes of Pomacea bridgesii fed on a lettuce diet and a hen's egg yolk diet, and snails starved for one week. beta-carotene and lutein concentrations were determined in the fecal matter of cultures on the lettuce and yolk diets as well. Significantly higher amounts of beta-carotene were found in the whole bodies of snails fed lettuce compared with those starved for a week. beta-carotene was not detected in the whole bodies and digestive gland-gonad complexes of snails fed hen's egg yolk. Neutral and polar lipid levels were also quantified in P. bridgesii fed lettuce and yolk. Significantly higher amounts of triacylglycerols were detected in the digestive gland-gonad complexes of snails maintained on the egg yolk diet.
采用高效薄层色谱法对以生菜和蛋黄为食的福寿螺以及饥饿一周的福寿螺的整个身体、消化腺-性腺复合体中的β-胡萝卜素和叶黄素浓度进行定量分析。同时也测定了以生菜和蛋黄为食的养殖福寿螺粪便中的β-胡萝卜素和叶黄素浓度。结果发现,与饥饿一周的福寿螺相比,喂食生菜的福寿螺体内β-胡萝卜素含量显著更高。在喂食蛋黄的福寿螺的整个身体和消化腺-性腺复合体中未检测到β-胡萝卜素。还对喂食生菜和蛋黄的福寿螺的中性和极性脂质水平进行了定量分析。结果发现,以蛋黄为食的福寿螺的消化腺-性腺复合体中三酰甘油含量显著更高。