Chen Wei, Li Lin, Gan Nanqin, Song Lirong
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, The Chinese Academy of Sciences, Wuhan 430072, P.R. China.
Environ Pollut. 2006 Sep;143(2):241-6. doi: 10.1016/j.envpol.2005.11.030. Epub 2006 Jan 18.
Microcystin analysis in sediments and soils is considered very difficult due to low recovery for extraction. This is the primary limiting factor for understanding the fate of toxins in the interface between water and sediment in both the aquatic ecosystem as well as in soils. In the present study, a wide range of extraction solvents were evaluated over a wide range of pH, extraction approaches and equilibration time to optimize an effective extraction procedure for the analysis of microcystins in soils and lake sediments. The number of extractions required and acids in extraction solutions were also studied. In this procedure, EDTA-sodium pyrophosphate solution was selected as an extraction solvent based on the adsorption mechanism study. The optimized procedure proved to be highly efficient and achieved over 90% recovery. Finally, the developed procedure was applied to field soil and sediment sample collected from Chinese lakes during bloom seasons and microcystins were determined in six of ten samples.
由于提取回收率低，沉积物和土壤中的微囊藻毒素分析被认为非常困难。这是了解水生生态系统以及土壤中水与沉积物界面中毒素归宿的主要限制因素。在本研究中，在广泛的pH值、提取方法和平衡时间范围内评估了多种提取溶剂，以优化用于分析土壤和湖泊沉积物中微囊藻毒素的有效提取程序。还研究了所需的提取次数和提取溶液中的酸。在此程序中，基于吸附机理研究，选择乙二胺四乙酸-焦磷酸钠溶液作为提取溶剂。优化后的程序证明效率很高，回收率超过90%。最后，将所开发的程序应用于中国湖泊在水华季节采集的田间土壤和沉积物样本，在十个样本中有六个样本检测到了微囊藻毒素。
