短链神经酰胺对双层结构域形成、厚度及链流动性的影响：二肉豆蔻酰磷脂酰胆碱（DMPC）与不对称神经酰胺混合物
Effects of a short-chain ceramide on bilayer domain formation, thickness, and chain mobililty: DMPC and asymmetric ceramide mixtures.
作者信息
Carrer Dolores C, Schreier Shirley, Patrito Martín, Maggio Bruno
机构信息
Departamento de Química Biológica-CIQUIBIC, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
出版信息
Biophys J. 2006 Apr 1;90(7):2394-403. doi: 10.1529/biophysj.105.074252. Epub 2006 Jan 20.
Abstract
An important part of natural ceramides contain asymmetric hydrocarbon chains. We have used calorimetry, atomic force microscopy, and electron paramagnetic resonance to study the effect of ceramide chain asymmetry in mixtures of C8Cer with DMPC as a model system of hydrocarbon chain disparity. A phase diagram is provided along with information on the thickness of the membrane and the mobility of the chains at different temperatures both below and above the phase transition temperature of the mixtures. The results indicate a partial interdigitation of C8Cer chains in the gel phase, producing a correlation between the organization of both hemilayers. Our data suggest that the effects of ceramides on biomembranes may be bimodal and similar to those of cholesterol.
摘要
天然神经酰胺的一个重要部分含有不对称的烃链。我们使用量热法、原子力显微镜和电子顺磁共振来研究C8神经酰胺与二肉豆蔻酰磷脂酰胆碱（DMPC）混合物中神经酰胺链不对称性的影响，该混合物作为烃链差异的模型系统。提供了一个相图，以及关于混合物相变温度以下和以上不同温度下膜厚度和链流动性的信息。结果表明，在凝胶相中C8神经酰胺链存在部分交叉，从而使两个半层的组织之间产生相关性。我们的数据表明，神经酰胺对生物膜的影响可能是双峰的，并且与胆固醇的影响相似。
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