Priestman D A, Mistry S C, Kerbey A L, Randle P J
Department of Clinical Biochemistry, University of Oxford, John Radcliffe Hospital, UK.
FEBS Lett. 1992 Aug 10;308(1):83-6. doi: 10.1016/0014-5793(92)81056-r.
Rat liver pyruvate dehydrogenase (PDH) kinase activator protein (KAP), a free PDH kinase readily separable from PDH complex and its intrinsic kinase, has been purified to apparent homogeneity from liver mitochondria of fed and 48-h starved rats. On SDS-PAGE an apparently single band of M(r) 45 kDa was obtained. N-Terminal amino acid sequence analyses (8-10 cycles) confirmed the presence of a single peptide in each case. The specific activity of the purified KAP from 48-h starved rats (14,413 U/mg protein) was 4.5-fold greater than that from fed rats.
Zotero 插件