Xi Jian-cheng, Ma Yuan-zheng, Zhou Bao-tong, Lin Hong-yuan, He Zhong-jie, Zhang Xing-huo, Fu Xiao-bing, Sheng Zhi-yong
Department of Orthopaedics, Second Affiliated Hospital of General Hospital of PLA, Beijing 100091, China.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2006 Jan;18(1):28-31.
To evaluate the changes in tumor necrosis factor-alpha (TNF-alpha) mRNA expression in peripheral polymorphonuclear leukocyte (PMNs) and tissues after cecal ligation puncture (CLP) in rats.
Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect TNF-alpha mRNA expression in peripheral PMNs and tissues.
The TNF-alpha mRNA expression in peripheral PMNs rose gradually after CLP, and it began to decrease after reaching the peak at 48 hours, but it was still higher than normal. The elevation of TNF-alpha mRNA expression was first limited in the regional tissues (P<0.01 at 12 hours after CLP and peaked at 24 hours in the intestine), then it entered the blood circulation later to affect the sensitive organs--lungs and livers (P<0.01 at 24 hours after CLP and peaked at 48 hours in the lung; P<0.05 at 24 hours after CLP and peaked at 48 hours in the liver).
The rise of TNF-alpha mRNA expression plays an important role in pathogenesis of sepsis.
评估大鼠盲肠结扎穿孔（CLP）后外周多形核白细胞（PMN）和组织中肿瘤坏死因子-α（TNF-α）mRNA表达的变化。
采用逆转录聚合酶链反应（RT-PCR）检测外周PMN和组织中TNF-αmRNA的表达。
CLP后外周PMN中TNF-αmRNA表达逐渐升高，在48小时达到峰值后开始下降，但仍高于正常水平。TNF-αmRNA表达的升高首先局限于局部组织（CLP后12小时P<0.01，在肠组织中24小时达到峰值），随后进入血液循环影响敏感器官——肺和肝脏（CLP后24小时P<0.01，在肺组织中48小时达到峰值；CLP后24小时P<0.05，在肝脏组织中48小时达到峰值）。
TNF-αmRNA表达的升高在脓毒症发病机制中起重要作用。
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